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Functionalized Fluorescent Nanostructures Generated from Self-Assembly of a Cationic Tripeptide Direct Cell-Selective Chemotherapeutic Drug Delivery.
Sivagnanam, Subramaniyam; Das, Kiran; Pan, Ieshita; Barik, Atanu; Stewart, Adele; Maity, Biswanath; Das, Priyadip.
Afiliación
  • Sivagnanam S; Department of Chemistry, SRM Institute of Science and Technology, SRM Nagar, Potheri, Kattankulathur 603203, Tamil Nadu, India.
  • Das K; Department of Systems Biology, Centre of Biomedical Research (CBMR), SGPGI Campus, Raebareli Road, Lucknow 226014, Uttar Pradesh, India.
  • Pan I; Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 602105, Tamil Nadu, India.
  • Barik A; Radiation & Photochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, Maharashtra, India.
  • Stewart A; Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Jupiter, Florida 33458, United States.
  • Maity B; Department of Systems Biology, Centre of Biomedical Research (CBMR), SGPGI Campus, Raebareli Road, Lucknow 226014, Uttar Pradesh, India.
  • Das P; Department of Chemistry, SRM Institute of Science and Technology, SRM Nagar, Potheri, Kattankulathur 603203, Tamil Nadu, India.
ACS Appl Bio Mater ; 6(2): 836-847, 2023 02 20.
Article en En | MEDLINE | ID: mdl-36757106
Nanodrug delivery systems (NDDs) capable of conveying chemotherapeutics directly into malignant cells without harming healthy ones are of significant interest in the field of cancer therapy. However, the development of nanostructures with the requisite biocompatibility, inherent optical properties, cellular penetration ability, encapsulation capability, and target selectivity has remained elusive. In an effort to develop cell-selective NDDs, we have synthesized a cationic tripeptide Boc-Arg-Trp-Phe-OMe (PA1), which self-assembles into well-ordered spheres in 100% aqueous medium. The inherent fluorescence properties of the peptide PA1 were shifted from the ultraviolet to the visible region by the self-assembly. These fluorescent nanostructures are proteolytically stable, photostable, and biocompatible, with characteristic blue fluorescence signals that permit us to monitor their intracellular entry in real time. We also demonstrate that these tripeptide spherical structures (TPSS) have the capacity to entrap the chemotherapeutic drug doxorubicin (Dox), shuttle the encapsulated drug within cancerous cells, and initiate the DNA damage signaling cascade, which culminates in apoptosis. Next, we functionalized the TPSS with an epithelial-cell-specific epithelial cell adhesion molecule aptamer. Aptamer-conjugated PA1 (PA1-Apt) facilitated efficient Dox delivery into the breast cancer epithelial cell line MCF7, resulting in cell death. However, cells of the human cardiomyocyte cell line AC16 were resistant to the cell killing actions of PA1-Apt. Together, these data demonstrate that not only can the self-assembly of cationic tripeptides like PA1 be exploited for efficient drug encapsulation and delivery but their unique chemistry also allows for functional modifications, which can improve the selectivity of these versatile NDDs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras / Nanopartículas Límite: Humans Idioma: En Revista: ACS Appl Bio Mater Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanoestructuras / Nanopartículas Límite: Humans Idioma: En Revista: ACS Appl Bio Mater Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos