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The role of AccCDK20 and AccCDKN1 from Apis cerana cerana in development and response to pesticide and heavy metal toxicity.
Bai, Jinhao; Guo, Dezheng; Li, Jing; Wang, Hongfang; Wang, Chen; Liu, Zhenguo; Guo, Xingqi; Wang, Ying; Xu, Baohua.
Afiliación
  • Bai J; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Guo D; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Li J; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Wang H; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Wang C; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Liu Z; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Guo X; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
  • Wang Y; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: wangying@sdau.edu.cn.
  • Xu B; College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: bhxu@sdau.edu.cn.
Pestic Biochem Physiol ; 190: 105333, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36740341
Apis cerana cerana is a native bee species in China and plays a key role in agricultural production and ecological balance. However, the growth and development of Apis cerana cerana has not been smooth, and pesticide and heavy metal stress are key factors that have forced a dramatic decline in population size. This study was performed with the objective of investigating the role of AccCDK20 and AccCDKN1 in honey bee resistance to pesticide and heavy metal stress. RT-qPCR analysis revealed that AccCDK20 transcript levels were highest in brown-eyed pupae and AccCDKN1 transcript levels were highest in 1-day-old worker bees. In different tissues and body parts of adult bees, AccCDK20 transcript levels were highest in the head, and AccCDKN1 transcript levels were highest in the thorax. It was further observed that environmental stress can affect the transcript levels of the AccCDK20 and AccCDKN1 genes. Silencing of the AccCDK20 and AccCDKN1 genes resulted in altered activities of antioxidant-related genes and antioxidant-related enzymes. AccCDK20 and AccCDKN1 transcript levels were upregulated under glyphosate stress, and silencing of the genes resulted in reduced resistance to glyphosate and greatly increased mortality in Apis cerana cerana. In addition, gene function was verified by in vitro repression assays. Overexpression of the AccCDK20 and AccCDKN1 proteins in E. coli cells increased the resistance to ROS damage induced by CHP. In conclusion, AccCDK20 and AccCDKN1 play an indispensable role in honey bee resistance to pesticide and heavy metal stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Pestic Biochem Physiol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Pestic Biochem Physiol Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos