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Tunable femtosecond laser suppresses the proliferation of breast cancer in vitro.
Taha, Safaa; Mohamed, Wafaa R; Elhemely, Mai A; El-Gendy, Ahmed O; Mohamed, Tarek.
Afiliación
  • Taha S; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt.
  • Mohamed WR; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt.
  • Elhemely MA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt.
  • El-Gendy AO; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt; Faculty of Pharmacy, Department of Microbiology and Immunology, Beni-Suef University, Beni-Suef 62514, Egypt.
  • Mohamed T; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt; Department of Engineering, Faculty of Advanced Technology and Multidiscipline, Universitas Airlangga, Indonesia. Electronic address: tarek_mohamed1969@lira.bsu.edu.eg.
J Photochem Photobiol B ; 240: 112665, 2023 Mar.
Article en En | MEDLINE | ID: mdl-36736031
Worldwide, the most frequently diagnosed cancer is female breast cancer, and it poses a serious global health threat. Traditional cancer therapies are associated with various side effects, so developing better therapies for breast cancer is necessary, such as laser therapy which could be a promising treatment option. The aim of the current study was to investigate the femtosecond laser irradiation effect on breast cancer using T47D cell line as an in vitro model. Cells were seeded at a density of 5 × 104 cells/well in 96-well plates and incubated overnight. After that, the cells were exposed to femtosecond laser irradiation at various wavelengths falling in the UV, visible, and IR ranges for 3, 5, or 10 min and at a constant power of 100 mW. Cell viability was measured directly and 24 h after femtosecond laser irradiation using MTT assay. When using different femtosecond laser irradiation parameters, especially the 380 and 400 nm femtosecond laser irradiation, there was significant inhibition of breast cancer cell growth, either directly or 24 h after femtosecond laser exposure. Also, 420 and 440 nm significantly affected the viability of the cells. It was also observed that increasing exposure time enhances the observed effect, so 10 min exposure time was the best time of exposure. However, 700, 720, 750, and 780 nm did not significantly affect the cells viability with different exposure times. It was possible to conclude from the aforementioned results that femtosecond laser irradiation exerted a significant anticancer effect against T47D cells. Consequently, the femtosecond laser could be used successfully for breast cancer management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Terapia por Luz de Baja Intensidad / Terapia por Láser Límite: Female / Humans Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Terapia por Luz de Baja Intensidad / Terapia por Láser Límite: Female / Humans Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza