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Rapid detection of serum free light chains by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Kaya, Zelal Zuhal; Serdar, Muhittin; Aksungar, Fehime; Kilercik, Meltem; Serteser, Mustafa; Baykal, Ahmet Tarik.
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  • Kaya ZZ; Department of Biochemistry and Molecular Biology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
  • Serdar M; Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
  • Aksungar F; Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
  • Kilercik M; Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
  • Serteser M; Department of Medical Biochemistry, Acibadem Labmed Clinical Laboratories, Istanbul, Turkey.
  • Baykal AT; Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
Eur J Mass Spectrom (Chichester) ; 29(2): 132-140, 2023 Apr.
Article en En | MEDLINE | ID: mdl-36734073
Introduction: Serum free light chain (FLC) measurements are increasingly prominent for patients with plasma cell disorders (PCDs) in screening, prognostic stratification, and monitoring therapy responses. Objectives: We aimed to develop a sensitive, reliable, and accurate method for diagnosing PCDs that can notably decrease the time and cost of current methods. Methods: Here, we present a novel approach for FLC measurement using immunoenrichment on micro-affinity chromatography in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) detection. In this study, serum free kappa (κ) and free lambda (λ) light chain (LC) levels in the serum of 105 patients were compared between the nephelometric serum FLC quantification and MALDI-TOF MS detection. Results: Cohen's kappa coefficient between the MALDI-TOF MS-based method and the FLC assay revealed an almost perfect agreement in the case of normal (negative) results (κ = 0.92; 95% confidence interval (CI): 0.837 to 0.968) and a good agreement in the case of increased (positive) results (κ = 0.76; 95% CI: 0.608 to 0.870). In Spearman's correlation analysis, the best correlation was found between serum free κ/λ ratios (r = 0.628, 0.496 to 0.732; p <0.0001). Our method showed sensitivity (92.5%) and specificity (76.3%) for discrimination between the κ/λ FLC ratio compared to the serum FLC assay. Conclusion: The proposed method can significantly contribute to diagnosing and monitoring PCDs as it can significantly be time-saving, cost-effective in FLC measurement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraproteinemias / Cadenas kappa de Inmunoglobulina Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Eur J Mass Spectrom (Chichester) Año: 2023 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraproteinemias / Cadenas kappa de Inmunoglobulina Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Eur J Mass Spectrom (Chichester) Año: 2023 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido