Your browser doesn't support javascript.
loading
Identification and characterization of pathogenicity-related genes of Rhizoctonia solani AG3 during tobacco infection.
Tang, Qianjun; Ma, Qianqian; Xiao, Zhipeng; Xiao, Yansong; Wang, Yunsheng; Liu, Lei; Peng, Weiye; Wang, Bing; Liu, Tianbo; Song, Na.
Afiliación
  • Tang Q; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Ma Q; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Xiao Z; Tobacco Research Institute of Hunan Provence, Changsha, Hunan, China.
  • Xiao Y; Tobacco Research Institute of Hunan Provence, Changsha, Hunan, China.
  • Wang Y; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Liu L; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Peng W; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Wang B; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
  • Liu T; Tobacco Research Institute of Hunan Provence, Changsha, Hunan, China.
  • Song N; College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
Front Plant Sci ; 13: 1116506, 2022.
Article en En | MEDLINE | ID: mdl-36733585
Tobacco target spot disease is caused by a ubiquitous soil-borne phytopathogen Rhizoctonia solani; the pathogenic mechanisms underlying the effects of R. solani remain unclear. Deeper understanding of the functional responses to R. solani during host plant infection would help identify the molecular mechanisms essential for successful host invasion. In this study, we performed global transcriptional analysis of R. solani during various stages (12, 24, 48, 72, 96, and 120 h) of tobacco infection via an RNA sequencing method, while utilizing the pathosystem model R. solani AG3-tobacco (Nicotiana tabacum L.). After R. solani inoculation, the number of differentially expressed genes of R. solani differed at the various time points. Moreover, several gene ontology and Kyoto encyclopedia of genes and genomes pathways were unique in different infection stages, especially with respect to the genes involved in plant cell wall degradation and catalysis of biotransformation reactions, such as the pectin metabolic process and pectin catabolic process. The overexpressing-PD8 N. benthamiana plants enhanced the susceptibility to R. solani. In addition, we found that large amounts of reactive oxygen species (ROS) were generated in tobacco after infected by R. solani. R. solani encoding FAD/NAD binding oxidoreductase and peroxidase gene family to eliminating ROS and counteract oxidative stress. Moreover, Perox3 was validated that can enhance the ability of scavenging ROS by co-injecting. Overall, our findings show that pectin-degrading enzymes and cytochrome P450 genes are critical for plant infection. These results provide comprehensive insights into R. solani AG3 transcriptome responses during tobacco invasion.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza