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Exosome-laden injectable self-healing hydrogel based on quaternized chitosan and oxidized starch attenuates disc degeneration by suppressing nucleus pulposus senescence.
Guan, Ming; Liu, Can; Zheng, Qiangqiang; Chu, Guangyu; Wang, Hehui; Jin, Jiale; Wu, Honghao; Chen, Jingyao; Huang, Qiong; Deng, Zexing; Wang, Yue.
Afiliación
  • Guan M; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
  • Liu C; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
  • Zheng Q; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou 310030, China.
  • Chu G; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
  • Wang H; Department of Orthopedics, Ningbo First Hospital, Ningbo 315000, China.
  • Jin J; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
  • Wu H; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
  • Chen J; Core Facilities, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • Huang Q; Core Facilities, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • Deng Z; College of Materials Science and Engineering, Xi'an University of Science and Technology, Xi'an 710054, China. Electronic address: biomaterial@xust.edu.cn.
  • Wang Y; Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. Electronic address: wangyuespine@zju.edu.cn.
Int J Biol Macromol ; 232: 123479, 2023 Mar 31.
Article en En | MEDLINE | ID: mdl-36731695
Disc degeneration is the common pathology underlying various degenerative spinal disorders and currently there is no effective cure. Here, we found nucleus pulposus (NP) cell senescence was closely associated with the severity of disc degeneration, and exosomes (Exos) derived from mesenchymal stem cells (MSCs) ameliorated NP cell senescence and promoted extracellular matrix (ECM) deposition. As chitosan-based hydrogels have been widely used as vehicles to deliver Exos due to their prominent antibacterial capacity, biocompatibility, and biodegradability, we developed an Exos-laden hydrogel based on quaternized chitosan (QCS) and oxidized starch (OST) to treat disc degeneration. The synthesized QCS-OST hydrogel is injectable, self-healing, biocompatible, and demonstrated desirable pore size, injectable properties, and sustainable release of Exos. In a rat model of disc degeneration, the QCS-OST/Exos hydrogel was able to rejuvenate NP cell senescence, promote ECM remodeling, and partially restore the structures of NP and annulus fibrosis. Our findings suggested that the novel QCS-OST/Exos hydrogel is an effective therapeutic strategy for treating disc degeneration via alleviating NP cell senescence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Exosomas / Degeneración del Disco Intervertebral / Núcleo Pulposo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitosano / Exosomas / Degeneración del Disco Intervertebral / Núcleo Pulposo Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos