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Evaluation of the Reveal® rapid AST system to assess the susceptibility of Pseudomonas aeruginosa from blood cultures.
Couchot, J; Fournier, D; Bour, M; Rajeev, L; Rhodes, P; Singh, P; Jeannot, K; Plésiat, P.
Afiliación
  • Couchot J; Laboratoire de Bactériologie, UMR 6249 CNRS ChronoEnvironnement, Université de Franche-Comté, 19 Rue Ambroise Paré, 25030, Besançon, France.
  • Fournier D; Centre National de Référence de La Résistance Aux Antibiotiques, Centre Hospitalier Universitaire, Besançon, France.
  • Bour M; Centre National de Référence de La Résistance Aux Antibiotiques, Centre Hospitalier Universitaire, Besançon, France.
  • Rajeev L; Specific Diagnostics, San José, USA.
  • Rhodes P; Specific Diagnostics, San José, USA.
  • Singh P; Specific Diagnostics, San José, USA.
  • Jeannot K; Centre National de Référence de La Résistance Aux Antibiotiques, Centre Hospitalier Universitaire, Besançon, France.
  • Plésiat P; Laboratoire de Bactériologie, UMR 6249 CNRS ChronoEnvironnement, Université de Franche-Comté, 19 Rue Ambroise Paré, 25030, Besançon, France. patrick.plesiat@univ-fcomte.fr.
Eur J Clin Microbiol Infect Dis ; 42(3): 359-363, 2023 Mar.
Article en En | MEDLINE | ID: mdl-36729319
This study was set up to assess the performance of the Reveal® rapid AST system to determine the drug susceptibility of Pseudomonas aeruginosa strains directly from blood cultures. Two hundred fully sequenced clinical P. aeruginosa strains were selected for the evaluation, of which 26.5% (n = 53) produced transferable ß-lactamases, and 2.0 to 33.0% had susceptibility levels close to the EUCAST 2021 breakpoints of 11 commonly used antipseudomonal antibiotics. The Reveal® AST system was run with a commercial MIC microplate designed for fast-growing Gram-negative bacilli (Microscan Neg MDR MIC 1), and was compared to the manually operated GN6F MIC microdilution panel from Thermo Fisher, as a comparator method. The Reveal® AST system provided MIC results for the 11 antipseudomonal antibiotics tested within a mean time to result of 6 h 22 min. By comparison with the GN6F panel, the overall rates of categorical agreement (CA), very major errors (VME), major errors (ME), and minor errors (mE for meropenem only) were 96.1%, 1.6%, 4.2%, and 0.6%, respectively. The Specific Reveal® AST system appears to be a reliable and fast technology to determine the susceptibility of P. aeruginosa to antibiotics, including those with resistance levels near categorical breakpoints, directly from blood cultures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Cultivo de Sangre Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Cultivo de Sangre Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania