Your browser doesn't support javascript.
loading
Distinct regulations driving YAP1 expression loss in poroma, porocarcinoma and RB1-deficient skin carcinoma.
Kervarrec, Thibault; Frouin, Eric; Collin, Christine; Tallet, Anne; Tallegas, Matthias; Pissaloux, Daniel; Tirode, Franck; Guyétant, Serge; Samimi, Mahtab; Gaboriaud, Pauline; Touzé, Antoine; Schrama, David; Houben, Roland; Tabareau-Delalande, Flore; Neuhart, Anne; de la Fouchardière, Arnaud; Osio, Amélie; Cavelier-Balloy, Bénédicte; Laurent-Roussel, Sara; Sohier, Pierre; Cyprien, Tilmant; Balme, Brigitte; Belzung, Fanny; Jullie, Marie-Laure; Cribier, Bernard; Battistella, Maxime; Macagno, Nicolas.
Afiliación
  • Kervarrec T; CARADERM, French Network of Rare Cutaneous Cancer, Lille, France.
  • Frouin E; Department of Pathology, University Hospital of Tours, Tours, France.
  • Collin C; 'Biologie des Infections à Polyomavirus' Team, UMR1282 INRAE, University of Tours, Tours, France.
  • Tallet A; CARADERM, French Network of Rare Cutaneous Cancer, Lille, France.
  • Tallegas M; Department of Pathology, University Hospital of Poitiers, University of Poitiers, LITEC, Poitiers, France.
  • Pissaloux D; Platform of Solid Tumor Molecular Genetics, University Hospital Center of Tours, Tours, France.
  • Tirode F; Platform of Solid Tumor Molecular Genetics, University Hospital Center of Tours, Tours, France.
  • Guyétant S; Platform of Solid Tumor Molecular Genetics, University Hospital Center of Tours, Tours, France.
  • Samimi M; Department of Biopathology, Center Léon Bérard, Lyon, France.
  • Gaboriaud P; University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, Equipe Labellisée Ligue contre le Cancer, Lyon, France.
  • Touzé A; Department of Biopathology, Center Léon Bérard, Lyon, France.
  • Schrama D; University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, Equipe Labellisée Ligue contre le Cancer, Lyon, France.
  • Houben R; Department of Pathology, University Hospital of Tours, Tours, France.
  • Tabareau-Delalande F; 'Biologie des Infections à Polyomavirus' Team, UMR1282 INRAE, University of Tours, Tours, France.
  • Neuhart A; Department of Pathology, University Hospital of Poitiers, University of Poitiers, LITEC, Poitiers, France.
  • de la Fouchardière A; Department of Dermatology, University Hospital Center of Tours, Tours, France.
  • Osio A; 'Biologie des Infections à Polyomavirus' Team, UMR1282 INRAE, University of Tours, Tours, France.
  • Cavelier-Balloy B; 'Biologie des Infections à Polyomavirus' Team, UMR1282 INRAE, University of Tours, Tours, France.
  • Laurent-Roussel S; Department of Dermatology, Venereology and Allergology, University Hospital Würzburg, Würzburg, Germany.
  • Sohier P; Department of Dermatology, Venereology and Allergology, University Hospital Würzburg, Würzburg, Germany.
  • Cyprien T; Department of Pathology, Hospital Centrer of Orléans, Orléans, France.
  • Balme B; Department of Biopathology, Center Léon Bérard, Lyon, France.
  • Belzung F; CARADERM, French Network of Rare Cutaneous Cancer, Lille, France.
  • Jullie ML; Department of Biopathology, Center Léon Bérard, Lyon, France.
  • Cribier B; University of Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Cancer Research Center of Lyon, Equipe Labellisée Ligue contre le Cancer, Lyon, France.
  • Battistella M; CARADERM, French Network of Rare Cutaneous Cancer, Lille, France.
  • Macagno N; Department of Pathology, Hospital Saint-Louis, AP-HP, Université Paris Cité, INSERM U976, Paris, France.
Histopathology ; 82(6): 885-898, 2023 May.
Article en En | MEDLINE | ID: mdl-36720791
AIMS: Recently, YAP1 fusion genes have been demonstrated in eccrine poroma and porocarcinoma, and the diagnostic use of YAP1 immunohistochemistry has been highlighted in this setting. In other organs, loss of YAP1 expression can reflect YAP1 rearrangement or transcriptional repression, notably through RB1 inactivation. In this context, our objective was to re-evaluate the performance of YAP1 immunohistochemistry for the diagnosis of poroma and porocarcinoma. METHODS AND RESULTS: The expression of the C-terminal part of the YAP1 protein was evaluated by immunohistochemistry in 543 cutaneous epithelial tumours, including 27 poromas, 14 porocarcinomas and 502 other cutaneous tumours. Tumours that showed a lack of expression of YAP1 were further investigated for Rb by immunohistochemistry and for fusion transcripts by real-time PCR (YAP1::MAML2 and YAP1::NUTM1). The absence of YAP1 expression was observed in 24 cases of poroma (89%), 10 porocarcinoma (72%), 162 Merkel cell carcinoma (98%), 14 squamous cell carcinoma (SCC) (15%), one trichoblastoma and one sebaceoma. Fusions of YAP1 were detected in only 16 cases of poroma (n = 66%), 10 porocarcinoma (71%) all lacking YAP1 expression, and in one sebaceoma. The loss of Rb expression was detected in all cases except one of YAP1-deficient SCC (n = 14), such tumours showing significant morphological overlap with porocarcinoma. In-vitro experiments in HaCat cells showed that RB1 knockdown resulted in repression of YAP1 protein expression. CONCLUSION: In addition to gene fusion, we report that transcriptional repression of YAP1 can be observed in skin tumours with RB1 inactivation, including MCC and a subset of SCC.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Neoplasias de las Glándulas Sudoríparas / Carcinoma / Poroma / Porocarcinoma Ecrino Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Histopathology Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Neoplasias de las Glándulas Sudoríparas / Carcinoma / Poroma / Porocarcinoma Ecrino Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Histopathology Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido