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MicroRNA-146a-5p and microRNA-210-3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population.
Eldosoky, Mona A; Hammad, Reham; Rushdi, Areej; Ibrahim, Hanan F; Tawfeik, Amany M; Mora, Ahmed; Fahmy, Sarah F; El-Ashmawy, Hossam; Ali, Elham; Hamed, Dina H; Mohammed, Amena Rezk; Mashaal, Alya; Mohsen, Hanan.
Afiliación
  • Eldosoky MA; Clinical Pathology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Hammad R; Clinical Pathology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Rushdi A; Microbiology and Immunology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Ibrahim HF; Microbiology and Immunology Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Tawfeik AM; Microbiology and Immunology Department, Faculty of Medicine, Badr University in Cairo (BUC), Cairo, Egypt.
  • Mora A; Chemistry Department, Faculty of Science (for Boys), Al-Azhar University, Cairo, Egypt.
  • Fahmy SF; Clinical Pharmacy Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
  • El-Ashmawy H; Clinical Pathology Department, Faculty of Medicine (for Boys), Al-Azhar University, Assuit, Egypt.
  • Ali E; Molecular Biology, Zoology and Entomology Department, Faculty of Science (For Girls), Al-Azhar University, Cairo, Egypt.
  • Hamed DH; Pediatric Department, Pediatric Allergy and Pulmonology Unit, Children's Hospital, Cairo University, Cairo, Egypt.
  • Mohammed AR; Biochemistry Department, Faculty of Medicine (for Girls), Al-Azhar University, Cairo, Egypt.
  • Mashaal A; Immunology, Zoology and Entomology Department, Faculty of Science (For Girls), Al-Azhar University, Cairo, Egypt.
  • Mohsen H; Pediatric Department, Pediatric Allergy and Pulmonology Unit, Children's Hospital, Cairo University, Cairo, Egypt.
J Asthma Allergy ; 16: 107-121, 2023.
Article en En | MEDLINE | ID: mdl-36714048
Background: Severe bronchial asthma (BA) affects 5-10% of children, which imposes socioeconomic burden. Therefore, it is crucial to identify biomarkers for risk stratification in children with BA. T regulatory cells (Tregs) play a balancing role in allergic response regulation. We aimed to investigate the relationship between Treg, miR-210-3p, and miR-146a-5p in relation to asthma phenotypes in search of novel biomarkers of disease severity. Methods: This study included 50 children with BA classified into Group 1 (n = 25) children with mild to moderate asthma and Group 2 (n = 25) children with severe asthma. In addition to 26 control subjects. Flow cytometry was used to detect Tregs. Plasma miR-210-3p and miR-146a levels were determined using quantitative real-time PCR. Patients' FEV1 (Forced Expiratory Volume in the first second) was measured. Results: miR-210-3p level correlated negatively with Treg frequency (r = -0.828, P < 0.001) and FEV1 (r = -0.621, P < 0.001). The level of miR-146a-5p positively correlated positively with Treg% (r = 0.303, P = 0.032). ROC curve analysis revealed that miR-210-3p was the most sensitive biomarker of severity, with the area under curve (AUC) = 0.923, 96% sensitivity, and 60% specificity. According to multivariate analysis, miR-210-3p is an independent risk factor for BA severity [OR =3.119, P = 0.030], while miR-146a-5p is a protective factor [OR =0.811, P = 0.049]. Conclusion: Treg frequency is linked to FEV1, miR-146a-5p and miR-210-3p in childhood BA. Upregulation of miR-210-3p is a sensitive biomarker and an independent risk factor for BA severity in Egyptian children.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Asthma Allergy Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Nueva Zelanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: J Asthma Allergy Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Nueva Zelanda