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Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers.
Mukherjee, Apratim; Ron, Jonathan Emanuel; Hu, Hooi Ting; Nishimura, Tamako; Hanawa-Suetsugu, Kyoko; Behkam, Bahareh; Mimori-Kiyosue, Yuko; Gov, Nir Shachna; Suetsugu, Shiro; Nain, Amrinder Singh.
Afiliación
  • Mukherjee A; Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.
  • Ron JE; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
  • Hu HT; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
  • Nishimura T; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
  • Hanawa-Suetsugu K; Graduate School of Brain Science, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
  • Behkam B; Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.
  • Mimori-Kiyosue Y; Laboratory for Molecular and Cellular Dynamics, RIKEN Center for Biosystems Dynamics Research, Minatojima-minaminachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
  • Gov NS; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 7610001, Israel.
  • Suetsugu S; Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
  • Nain AS; Data Science Center, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
Adv Sci (Weinh) ; 10(7): e2207368, 2023 03.
Article en En | MEDLINE | ID: mdl-36698307
The cell migration cycle, well-established in 2D, proceeds with forming new protrusive structures at the cell membrane and subsequent redistribution of contractile machinery. Three-dimensional (3D) environments are complex and composed of 1D fibers, and 1D fibers are shown to recapitulate essential features of 3D migration. However, the establishment of protrusive activity at the cell membrane and contractility in 1D fibrous environments remains partially understood. Here the role of membrane curvature regulator IRSp53 is examined as a coupler between actin filaments and plasma membrane during cell migration on single, suspended 1D fibers. IRSp53 depletion reduced cell-length spanning actin stress fibers that originate from the cell periphery, protrusive activity, and contractility, leading to uncoupling of the nucleus from cellular movements. A theoretical model capable of predicting the observed transition of IRSp53-depleted cells from rapid stick-slip migration to smooth and slower migration due to reduced actin polymerization at the cell edges is developed, which is verified by direct measurements of retrograde actin flow using speckle microscopy. Overall, it is found that IRSp53 mediates actin recruitment at the cellular tips leading to the establishment of cell-length spanning fibers, thus demonstrating a unique role of IRSp53 in controlling cell migration in 3D.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Movimiento Celular / Actinas Tipo de estudio: Prognostic_studies Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto de Actina / Movimiento Celular / Actinas Tipo de estudio: Prognostic_studies Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania