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Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR-145-5p/TGFB2 axis.
Song, Meiyu; Xu, Peng; Wang, Li; Liu, Jie; Hou, Xiaofei.
Afiliación
  • Song M; Department of Obstetrics, Yantai Yantaishan Hospital, Yantai, Shandong, China.
  • Xu P; Department of Nursing, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
  • Wang L; Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
  • Liu J; Department of Obstetrics, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
  • Hou X; Department of Clinical Laboratory, Yantai Yuhuangding Hospital, Yantai, Shandong, China.
Am J Reprod Immunol ; 89(5): e13682, 2023 05.
Article en En | MEDLINE | ID: mdl-36670490
PROBLEM: Preeclampsia (PE) is an obstetric disease involving multiple systems, which account for maternal and fetal complications and increased mortality. Circular RNAs (circRNAs) were recently deemed to associate with the pathogenesis of PE. This study aims to clarify the correlation between circRNA hsa_circ_0001326 and PE and explore its biological function in PE. METHOD OF STUDY: The expression of hsa_circ_0001326 in PE placentas was detected by real-time quantitative PCR (qRT-PCR). After overexpressing or inhibiting hsa_circ_0001326 in trophoblast cells, the cell growth, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8) and transwell assays. Western blot assay was applied to detect the epithelial-mesenchymal transition (EMT) proteins, E-cadherin and Vimentin. Furthermore, a dual-luciferase reporter assay was applied to verify the binding sites of hsa_circ_0001326, miR-145-5p, and transforming growth factor beta 2 (TGFB2). RESULTS: Hsa_circ_0001326 was found to be higher expressed in PE placentas than in normal placentas. Furthermore, hsa_circ_0001326 played a negative regulating role in trophoblast cell viability, migration, and invasion. Overexpression of hsa_circ_0001326 inhibited the viability, migration, and invasion of trophoblast cells, while inhibition of hsa_circ_0001326 showed opposite effects. Mechanistically, hsa_circ_0001326 sponged miR-145-5p to elevate TGFB2 expression in trophoblast cells. CONCLUSION: This study provided evidence that the up-regulated hsa_circ_0001326 in PE restrained trophoblast cells proliferation, migration, and invasion by sponging miR-145-5p to elevate TGFB2 expression. Our results might provide a novel insight into the role of hsa_circ_0001326 in the pathogenesis of PE.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / MicroARNs / Factor de Crecimiento Transformador beta2 / ARN Circular Límite: Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Dinamarca
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / MicroARNs / Factor de Crecimiento Transformador beta2 / ARN Circular Límite: Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Dinamarca