Your browser doesn't support javascript.
loading
A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport.
Klee, Katharina M C; Hess, Michael W; Lohmüller, Michael; Herzog, Sebastian; Pfaller, Kristian; Müller, Thomas; Vogel, Georg F; Huber, Lukas A.
Afiliación
  • Klee KMC; Institute of Cell Biology, Medical University of Innsbruck, Innsbruck, Austria.
  • Hess MW; Institute of Histology and Embryology, Medical University of Innsbruck, Innsbruck, Austria.
  • Lohmüller M; Institute of Histology and Embryology, Medical University of Innsbruck, Innsbruck, Austria.
  • Herzog S; Institute of Developmental Immunology, Medical University of Innsbruck, Innsbruck, Austria.
  • Pfaller K; Institute of Developmental Immunology, Medical University of Innsbruck, Innsbruck, Austria.
  • Müller T; Institute of Histology and Embryology, Medical University of Innsbruck, Innsbruck, Austria.
  • Vogel GF; Department of Paediatrics I, Medical University of Innsbruck, Innsbruck, Austria.
  • Huber LA; Institute of Cell Biology, Medical University of Innsbruck, Innsbruck, Austria.
Elife ; 122023 01 20.
Article en En | MEDLINE | ID: mdl-36661306
Epithelial polarization and polarized cargo transport are highly coordinated and interdependent processes. In our search for novel regulators of epithelial polarization and protein secretion, we used a genome-wide CRISPR/Cas9 screen and combined it with an assay based on fluorescence-activated cell sorting (FACS) to measure the secretion of the apical brush-border hydrolase dipeptidyl peptidase 4 (DPP4). In this way, we performed the first CRISPR screen to date in human polarized epithelial cells. Using high-resolution microscopy, we detected polarization defects and mislocalization of DPP4 to late endosomes/lysosomes after knockout of TM9SF4, anoctamin 8, and ARHGAP33, confirming the identification of novel factors for epithelial polarization and apical cargo secretion. Thus, we provide a powerful tool suitable for studying polarization and cargo secretion in epithelial cells. In addition, we provide a dataset that serves as a resource for the study of novel mechanisms for epithelial polarization and polarized transport and facilitates the investigation of novel congenital diseases associated with these processes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dipeptidil Peptidasa 4 / Células Epiteliales Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Elife Año: 2023 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dipeptidil Peptidasa 4 / Células Epiteliales Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Elife Año: 2023 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Reino Unido