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A novel and ultrasensitive electrochemical DNA biosensor for pralatrexate detection.
Ghasemi, Leila; Jahani, Shohreh; Ghazizadeh, Mahdiyeh; Foroughi, Mohammad Mehdi.
Afiliación
  • Ghasemi L; Department of Chemistry, Islamic Azad University, Kerman Branch, Kerman, Iran. foroughi@iauk.ac.ir.
  • Jahani S; Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran.
  • Ghazizadeh M; Department of Chemistry, Islamic Azad University, Kerman Branch, Kerman, Iran. foroughi@iauk.ac.ir.
  • Foroughi MM; Department of Chemistry, Islamic Azad University, Kerman Branch, Kerman, Iran. foroughi@iauk.ac.ir.
Anal Methods ; 15(5): 631-638, 2023 02 02.
Article en En | MEDLINE | ID: mdl-36651313
An attempt was made to develop a new sensitive biosensor for pralatrexate, as an anticancer drug, based on its interaction with the guanine of fish sperm DNA anchored on a screen-printed electrode (SPE) modified with polypyrrole (PP)/octahedral Pd-doped Co3O4 composite (Oh-Pd-doped Co3O4 C). Electrochemical techniques like differential pulse voltammetry verified the mechanism of such an interaction on the dsDNA/PP/Oh-Pd-doped Co3O4 C/SPE surface. A reduction in the peak current of guanine oxidation elucidated the interaction in acetate buffer with pH = 4.8. The optimization of response was performed for the interaction method according to potential, accumulation time, reproducibility and drug content. The linear dynamic range was estimated at 1.0 nM to 150.0 µM as well as a limit of detection as low as 0.61 nM for the DNA and pralatrexate concentrations. The practical potential of the proposed sensor was verified by determining pralatrexate in its pharmaceutical matrices.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas Biosensibles Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Methods Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas Biosensibles Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Methods Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido