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Optimization of C50 Carotenoids Production by Open Fermentation of Halorubrum sp. HRM-150.
Ma, Ying-Chao; Gao, Mei-Rong; Yang, Huan; Jiang, Jun-Yao; Xie, Wei; Su, Wan-Ping; Zhang, Bo; Yeong, Yik-Sung; Guo, Wu-Yan; Sui, Li-Ying.
Afiliación
  • Ma YC; Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education, Tianjin, China.
  • Gao MR; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Yang H; Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, China.
  • Jiang JY; Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education, Tianjin, China.
  • Xie W; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Su WP; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Zhang B; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Yeong YS; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Guo WY; Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin, 300457, China.
  • Sui LY; Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, China.
Appl Biochem Biotechnol ; 195(6): 3628-3640, 2023 Jun.
Article en En | MEDLINE | ID: mdl-36648604
C50 carotenoids, as unique bioactive molecules, have many biological properties, including antioxidant, anticancer, and antibacterial activity, and have a wide range of potential uses in the food, cosmetic, and biomedical industries. The majority of C50 carotenoids are produced by the sterile fermentation of halophilic archaea. This study aims to look at more cost-effective and manageable ways of producing C50 carotenoids. The basic medium, carbon source supplementation, and optimal culture conditions for Halorubrum sp. HRM-150 C50 carotenoids production by open fermentation were examined in this work. The results indicated that Halorubrum sp. HRM-150 grown in natural brine medium grew faster than artificial brine medium. The addition of glucose, sucrose, and lactose (10 g/L) enhanced both biomass and carotenoids productivity, with the highest level reaching 4.53 ± 0.32 µg/mL when glucose was added. According to the findings of orthogonal studies based on the OD600 and carotenoids productivity, the best conditions for open fermentation were salinity 20-25%, rotation speed 150-200 rpm, and pH 7.0-8.2. The up-scaled open fermentation was carried out in a 7 L medium under optimum culture conditions. At 96 h, the OD600 and carotenoids productivity were 9.86 ± 0.51 (dry weight 10.40 ± 1.27 g/L) and 7.31 ± 0.65 µg/mL (701.40 ± 21.51 µg/g dry weight, respectively). When amplified with both universal bacterial primer and archaeal primer in the open fermentation, Halorubrum remained the dominating species, indicating that contamination was kept within an acceptable level. To summarize, open fermentation of Halorubrum is a promising method for producing C50 carotenoids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Halorubrum Idioma: En Revista: Appl Biochem Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Halorubrum Idioma: En Revista: Appl Biochem Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos