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Simultaneous identification of viruses and viral variants with programmable DNA nanobait.
Boskovic, Filip; Zhu, Jinbo; Tivony, Ran; Ohmann, Alexander; Chen, Kaikai; Alawami, Mohammed F; Dordevic, Milan; Ermann, Niklas; Pereira-Dias, Joana; Fairhead, Michael; Howarth, Mark; Baker, Stephen; Keyser, Ulrich F.
Afiliación
  • Boskovic F; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Zhu J; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Tivony R; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Ohmann A; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Chen K; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Alawami MF; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Dordevic M; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Ermann N; Cavendish Laboratory, University of Cambridge, Cambridge, UK.
  • Pereira-Dias J; University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Hills Road, Cambridge, UK.
  • Fairhead M; Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Hills Road, Cambridge, UK.
  • Howarth M; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Baker S; Department of Biochemistry, University of Oxford, Oxford, UK.
  • Keyser UF; University of Cambridge School of Clinical Medicine, Cambridge Biomedical Campus, Hills Road, Cambridge, UK.
Nat Nanotechnol ; 18(3): 290-298, 2023 03.
Article en En | MEDLINE | ID: mdl-36646828
Respiratory infections are the major cause of death from infectious disease worldwide. Multiplexed diagnostic approaches are essential as many respiratory viruses have indistinguishable symptoms. We created self-assembled DNA nanobait that can simultaneously identify multiple short RNA targets. The nanobait approach relies on specific target selection via toehold-mediated strand displacement and rapid readout via nanopore sensing. Here we show that this platform can concurrently identify several common respiratory viruses, detecting a panel of short targets of viral nucleic acids from multiple viruses. Our nanobait can be easily reprogrammed to discriminate viral variants with single-nucleotide resolution, as we demonstrated for several key SARS-CoV-2 variants. Last, we show that the nanobait discriminates between samples extracted from oropharyngeal swabs from negative- and positive-SARS-CoV-2 patients without preamplification. Our system allows for the multiplexed identification of native RNA molecules, providing a new scalable approach for the diagnostics of multiple respiratory viruses in a single assay.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Nat Nanotechnol Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Nat Nanotechnol Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido