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Ergot alkaloids in sclerotia collected in Japan: synthetic profiles and induction of apoptosis by Clavine-type compounds.
Doi, Yuji; Wakana, Daigo; Kitaoka, Satoshi; Sato, Fumiaki; Tanaka, Eiji; Takeda, Hisashi; Hosoe, Tomoo.
Afiliación
  • Doi Y; Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan.
  • Wakana D; Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan.
  • Kitaoka S; Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
  • Sato F; Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan.
  • Tanaka E; Department of Environmental Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan.
  • Takeda H; Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan.
  • Hosoe T; Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa, Tokyo, Japan. hosoe@hoshi.ac.jp.
J Nat Med ; 77(2): 306-314, 2023 Mar.
Article en En | MEDLINE | ID: mdl-36635416
The genus Claviceps (Clavicipitaceae) is famous for producing ergot alkaloids (EAs) in sclerotia. EAs can cause ergotism, resulting in convulsions and necrosis when ingested, making these compounds a serious concern for food safety. Agroclavine (2), a typical Clavine-type EA, is a causative agent of ergotism and is listed as a compound to be monitored by the European Food Safety Authority. Clavine-type EAs are known to cause cytotoxicity, but the mechanism has not been elucidated. We performed annexin V and PI double-staining followed by flow cytometric analysis to detect apoptosis in HepG2 and PANC-1 cells after exposure to Clavine-type EAs. Clavine-type EAs reduced cell viability and induced apoptosis in both cell lines. We then performed LC-MS analysis of EAs from 41 sclerotia samples of Claviceps collected in Japan. 24 out of 41 sclerotia extracts include peptide-type EAs (ergosine/inine: 4/4', ergotamine: 5, ergocornine/inine: 6/6', α-ergocryptine/inine: 8/8', and ergocristine/inine: 9/9') and 19 sclerotia extracts among 24 sclerotia detected peptide type EAs include Clavine-type EAs (pyroclavine: 1, agroclavine: 2, festuclavine: 3) by LC-MS. We then performed a metabolomic analysis of the EAs in the sclerotia using principal component analysis (PCA). The PCA score plots calculated for EAs suggested the existence of four groups with different EA production patterns. One of the groups was formed by the contribution of Clavine-type EAs. These results suggest that Clavine-type EAs are a family of compounds requiring attention in food safety and livestock production in Japan.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ergotismo / Claviceps / Alcaloides de Claviceps Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Nat Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ergotismo / Claviceps / Alcaloides de Claviceps Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Nat Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón