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A simplified courtship conditioning protocol to test learning and memory in Drosophila.
Gil-Martí, Beatriz; Barredo, Celia G; Pina-Flores, Sara; Poza-Rodriguez, Adriana; Treves, Gaia; Rodriguez-Navas, Carmen; Camacho, Lucía; Pérez-Serna, Atenea; Jimenez, Iñaki; Brazales, Laura; Fernandez, Javier; Martin, Francisco A.
Afiliación
  • Gil-Martí B; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain; Department of Biology, Autonomous University of Madrid, Madrid, Spain. Electronic address: bgilmarti@cajal.csic.es.
  • Barredo CG; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Pina-Flores S; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Poza-Rodriguez A; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Treves G; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Rodriguez-Navas C; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Camacho L; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Pérez-Serna A; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Jimenez I; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Brazales L; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Fernandez J; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain.
  • Martin FA; Cajal Institute, Spanish National Research Council (CSIC), Av Dr Arce 37, 28002 Madrid, Spain. Electronic address: famartin@cajal.csic.es.
STAR Protoc ; 4(1): 101572, 2023 03 17.
Article en En | MEDLINE | ID: mdl-36633946
In Drosophila, a male that has previously been sexually rejected reduces its courtship behavior when confronted again with an unreceptive female. This reduced courting time reflects a memory formation process. Here, we describe a simplified protocol to perform the courtship conditioning assay for assessing the reduced courting time, using regular lab equipment and handmade tools. Every step of the procedure, from raising flies and training to testing and quantification of this memory-related behavior, can be implemented in any practice laboratory.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cortejo / Drosophila Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cortejo / Drosophila Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos