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Assembly and annotation of the Gossypium barbadense L. 'Pima-S6' genome raise questions about the chromosome structure and gene content of Gossypium barbadense genomes.
Chávez Montes, Ricardo A; Ulloa, Mauricio; Biniashvili, Tammy; Zackay, Arik; Kfir, Nir; Lopez-Arredondo, Damar; Herrera-Estrella, Luis.
Afiliación
  • Chávez Montes RA; Institute of Genomics for Crop Abiotic Stress Tolerance, Plant and Soil Science Department, Texas Tech University, 79409, Lubbock, TX, USA.
  • Ulloa M; Plant Stress and Germplasm Development Research, USDA-ARS, PA, CSRL, 79415, Lubbock, TX, USA. mauricio.ulloa@usda.gov.
  • Biniashvili T; NRGene, Ness-Ziona, Israel.
  • Zackay A; NRGene, Ness-Ziona, Israel.
  • Kfir N; NRGene, Ness-Ziona, Israel.
  • Lopez-Arredondo D; Institute of Genomics for Crop Abiotic Stress Tolerance, Plant and Soil Science Department, Texas Tech University, 79409, Lubbock, TX, USA. Damar.Lopez-Arredondo@ttu.edu.
  • Herrera-Estrella L; Institute of Genomics for Crop Abiotic Stress Tolerance, Plant and Soil Science Department, Texas Tech University, 79409, Lubbock, TX, USA. Luis.Herrera-Estrella@ttu.edu.
BMC Genomics ; 24(1): 11, 2023 Jan 10.
Article en En | MEDLINE | ID: mdl-36627552
BACKGROUND: Gossypium barbadense L. Pima cotton is known for its resistance to Fusarium wilt and for producing fibers of superior quality highly prized in the textile market. We report a high-quality genome assembly and annotation of Pima-S6 cotton and its comparison at the chromosome and protein level to other ten Gossypium published genome assemblies. RESULTS: Synteny and orthogroup analyses revealed important differences on chromosome structure and annotated proteins content between our Pima-S6 and other publicly available G. barbadense assemblies, and across Gossypium assemblies in general. Detailed synteny analyses revealed chromosomal rearrangements between Pima-S6 and other Pima genomes on several chromosomes, with three major inversions in chromosomes A09, A13 and D05, raising questions about the true chromosome structure of Gossypium barbadense genomes. CONCLUSION: Analyses of the re-assembled and re-annotated genome of the close relative G. barbadense Pima 3-79 using our Pima-S6 assembly suggest that contig placement of some recent G. barbadense assemblies might have been unduly influenced by the use of the G. hirsutum TM-1 genome as the anchoring reference. The Pima-S6 reference genome provides a valuable genomic resource and offers new insights on genomic structure, and can serve as G. barbadense genome reference for future assemblies and further support FOV4-related studies and breeding efforts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yoduro de Potasio / Gossypium Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yoduro de Potasio / Gossypium Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido