A4, Androstendione; ALDO, Aldosterone; APCI, Atmospheric pressure chemical ionization; CAH, Congenital adrenal hyperplasia; CE, Collision energy; CE-IVD, Certified in-vitro-diagnostic device; CV, Coefficient of variation; DHEA, Dehydro-epiandrosterone; DHEA-S, Dehydro-epiandrosterone sulfate; DHT, Dihydrotestosterone; DOC, 11-deoxycorticosterone; E, Cortisone; E2, Estradiol; EMA, European Medicines Agency; EQA, External quality assessment; ESI, Electrospray ionisation; F, Cortisol; IVD, In-vitro-diagnostic; IVDR, EU In vitro Diagnostic Regulation; LC, Liquid chromatography; LC­MS/MS, Liquid chromatography tandem mass spectrometry; LDT, Laboratory developed test; LLOQ, Lower limit of quantification; MRM, Multiple reaction monitoring; On-line solid phase extraction (SPE); P4, Progesterone; QC, Quality control; Robustness; S/N, Signal-to-noise ratio; SID, Stable-isotope dilution; SPE, Solid phase extraction; SST, System suitability test; Serum steroid profiling; Stable-isotope dilution liquid chromatography-tandem mass spectrometry (SID LC-MS/MS); UHPLC, Ultra high performance liquid chromatography; ULOQ, Upper limit of quantification