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Oocyte developmental capacity is influenced by intrinsic ovarian factors in a bovine model for individual embryo production.
Azari-Dolatabad, Nima; Benedetti, Camilla; Velez, Daniel Angel; Montoro, Andrea Fernandez; Sadeghi, Hafez; Residiwati, Gretania; Leroy, Jo L M R; Van Soom, Ann; Pascottini, Osvaldo Bogado.
Afiliación
  • Azari-Dolatabad N; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Department of Veterinary Sciences, Gamete Research Center, Veterinary Physiology and Biochemistry, University of Antwerp, Wilrijk, Belgium. Electronic address:
  • Benedetti C; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Velez DA; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Research Group in Animal Sciences - INCA-CES, School of Veterinary Medicine and Animal Production, Universidad CES, Medellin, Colombia.
  • Montoro AF; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Sadeghi H; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Residiwati G; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Leroy JLMR; Department of Veterinary Sciences, Gamete Research Center, Veterinary Physiology and Biochemistry, University of Antwerp, Wilrijk, Belgium.
  • Van Soom A; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Pascottini OB; Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Department of Veterinary Sciences, Gamete Research Center, Veterinary Physiology and Biochemistry, University of Antwerp, Wilrijk, Belgium.
Anim Reprod Sci ; 249: 107185, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36610102
The ovary and its hormones may have major effects on the in vitro developmental capacity of the oocytes it contains. We related intrinsic ovarian factors namely the presence of corpus luteum (CL) and/or dominant follicle (>8 mm) and the follicular count to cumulus expansion (CE), embryo development, and blastocyst quality in a bovine model. Cumulus-oocyte-complexes (COCs) were aspirated from follicles between 4 and 8 mm in diameter. In vitro embryo production was performed in a fully individual production system. The follicular fluid from which COCs were collected was pooled (per ovary) to evaluate the estrogen, progesterone, and insulin-like growth factor-1 (IGF-1) concentrations. Cumulus oocyte complexes collected from ovaries without a CL presented a greater CE than COCs derived from ovaries bearing CL. The absence of ovarian structures increased the blastocyst rate when compared to oocytes derived from ovaries with a CL, a dominant follicle, or both. Blastocysts derived from ovaries without a dominant follicle presented higher total cell numbers and a lower proportion of apoptosis than blastocysts derived from ovaries containing a dominant follicle. Cumulus oocyte complexes collected from ovaries with high follicular count resulted in higher cleavage than from ovaries with low follicular count, but the blastocyst rate was similar between groups. Ovaries bearing a CL had greater progesterone and IGF-1 follicular fluid concentrations in neighboring follicles than ovaries without a CL. Selection for bovine ovaries without CL or dominant follicle can have positive effects on CE, embryo development, and blastocyst quality in an individual embryo production system set-up.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Factor I del Crecimiento Similar a la Insulina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Factor I del Crecimiento Similar a la Insulina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Anim Reprod Sci Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos