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An assay to evaluate the capacity of cholesterol acceptors using BODIPY-cholesterol in cells.
Raulin, Ana-Caroline; Liu, Chia-Chen; Bu, Guojun.
Afiliación
  • Raulin AC; Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: raulin.ana-caroline@mayo.edu.
  • Liu CC; Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: liu.chiachen@mayo.edu.
  • Bu G; Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: guojun.bu@molecularneurodegeneration.org.
STAR Protoc ; 4(1): 101976, 2023 03 17.
Article en En | MEDLINE | ID: mdl-36598853
Cholesterol is a structural component of cell membranes. Most cells are incapable of its catabolism, and intracellular cholesterol accumulation is linked to several disorders including cardiovascular and neurodegenerative diseases. Cholesterol efflux, essential to its metabolism, is dependent on acceptors such as apolipoproteins. Here, we describe an assay to evaluate the capacity of cholesterol acceptors. Cells are treated with an analog of cholesterol tagged with fluorescent BODIPY. Addition of an acceptor leads to BODIPY-cholesterol efflux, measured using a plate reader. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apolipoproteínas / Colesterol Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apolipoproteínas / Colesterol Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos