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PHF20 is a Novel Prognostic Biomarker and Correlated with Immune Status in Breast Cancer.
Chen, Zhiyuan; Zhong, Shanliang; Zhang, Zhengdong; Tang, Jinhai.
Afiliación
  • Chen Z; Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, China.
  • Zhong S; Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
  • Zhang Z; Center of Clinical Laboratory Science, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.
  • Tang J; Department of Environmental Genomics, School of Public Health, Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangning District, China. drzdzhang@njmu.edu.cn.
Biochem Genet ; 61(4): 1369-1386, 2023 Aug.
Article en En | MEDLINE | ID: mdl-36598702
Increasing evidence has demonstrated that enhanced PHF20 expression plays a crucial role in cancer development and progression. However, little is known about the prognostic value of PHF20 expression in breast cancer (BRCA). In this study, we attempted to explore the expression pattern of PHF20 and its associations with prognosis and immune status in BRCA. The gene expression data and clinical information of 1109 BRCA samples were downloaded from TCGA database. The expression level of PHF20 protein was determined using UALCAN and HPA database. Kaplan-Meier method and CIBERSORT algorithm were used to analyze the associations of PHF20 expression with overall survival (OS) and immune microenvironment, respectively. Besides, GSEA analysis was conducted to explore potential biological functions and molecular mechanisms of PHF20 in BRCA. Moreover, starBase database was applied to construct a ceRNA nework. PHF20 was highly expressed in BRCA samples both at the transcriptional and protein level, and was strongly correlated with the OS and immune status. Univariable and multivariate Cox regression analyses identified PHF20 as an independent prognostic factor. Additionally, GSEA analysis showed that high PHF20 expression was closely associated with TGF-ß signaling pathway, Wnt signaling pathway, and adherens junction. Furthermore, three ceRNA networks (AC037198.1/hsa-miR-223-3p/PHF20, CBR3-AS1/hsa-miR-223-3p/PHF20, and ZNF561-AS1/hsa-miR-223-3p/PHF20) were identified by starBase analysis. Functional experiments validated that PHF20 knockdown inhibited the cell viability and progression in BRCA cells. PHF20 overexpression was significantly associated with poor prognosis and immune status in BRCA, and could act as a potential novel prognostic biomarker for BRCA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Neoplasias Tipo de estudio: Prognostic_studies Idioma: En Revista: Biochem Genet Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Neoplasias Tipo de estudio: Prognostic_studies Idioma: En Revista: Biochem Genet Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos