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Metaproteomic and gene expression analysis of interspecies interactions in a PAH-degrading synthetic microbial consortium constructed with the key microbes of a natural consortium.
Nieto, Esteban E; Macchi, Marianela; Valacco, María P; Festa, Sabrina; Morelli, Irma S; Coppotelli, Bibiana M.
Afiliación
  • Nieto EE; Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Street 50 N°227, 1900, La Plata, Argentina.
  • Macchi M; Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Street 50 N°227, 1900, La Plata, Argentina.
  • Valacco MP; Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales IQUIBICEN, FCEN-UBA, Buenos Aires, Argentina.
  • Festa S; Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Street 50 N°227, 1900, La Plata, Argentina.
  • Morelli IS; Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET), Street 50 N°227, 1900, La Plata, Argentina.
  • Coppotelli BM; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, La Plata, Argentina.
Biodegradation ; 34(2): 181-197, 2023 04.
Article en En | MEDLINE | ID: mdl-36596914
Polycyclic Aromatic Hydrocarbons (PAHs) impose adverse effects on the environment and human life. The use of synthetic microbial consortia is promising in bioremediation of contaminated sites with these pollutants. However, the design of consortia taking advantage of natural interactions has been poorly explored. In this study, a dual synthetic bacterial consortium (DSC_AB) was constructed with two key members (Sphingobium sp. AM and Burkholderia sp. Bk), of a natural PAH degrading consortium. DSC_AB showed significantly enhanced degradation of PAHs and toxic intermediary metabolites relative to the axenic cultures, indicating the existence of synergistic relationships. Metaproteomic and gene-expression analyses were applied to obtain a view of bacterial performance during phenanthrene removal. Overexpression of the Bk genes, naph, biph, tol and sal and the AM gene, ahdB, in DSC_AB relative to axenic cultures, demonstrated that both strains are actively participating in degradation, which gave evidence of cross-feeding. Several proteins related to stress response were under-expressed in DSC_AB relative to axenic cultures, indicating that the division of labour reduces cellular stress, increasing the efficiency of degradation. This is the one of the first works revealing bacterial relationships during PAH removal in a synthetic consortium applying an omics approach. Our findings could be used to develop criteria for evaluating the potential effectiveness of synthetic bacterial consortia in bioremediation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes del Suelo / Sphingomonadaceae Límite: Humans Idioma: En Revista: Biodegradation Asunto de la revista: BIOQUIMICA / SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes del Suelo / Sphingomonadaceae Límite: Humans Idioma: En Revista: Biodegradation Asunto de la revista: BIOQUIMICA / SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Países Bajos