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Collagen-chitosan-hydroxyapatite composite scaffolds for bone repair in ovariectomized rats.
Chacon, Erivelto Luís; Bertolo, Mirella Romanelli Vicente; de Guzzi Plepis, Ana Maria; da Conceição Amaro Martins, Virginia; Dos Santos, Geovane Ribeiro; Pinto, Clovis Antônio Lopes; Pelegrine, André Antônio; Teixeira, Marcelo Lucchesi; Buchaim, Daniela Vieira; Nazari, Fabricio Montenegro; Buchaim, Rogerio Leone; Sugano, Gustavo Tenório; da Cunha, Marcelo Rodrigues.
Afiliación
  • Chacon EL; Department of Morphology and Pathology, Jundiai Medical School, Jundiai, 13202-550, Brazil.
  • Bertolo MRV; Sao Carlos Institute of Chemistry, University of Sao Paulo (USP), Sao Carlos, 13566-590, Brazil.
  • de Guzzi Plepis AM; Sao Carlos Institute of Chemistry, University of Sao Paulo (USP), Sao Carlos, 13566-590, Brazil.
  • da Conceição Amaro Martins V; Interunits Graduate Program in Bioengineering (EESC/FMRP/IQSC), University of Sao Paulo (USP), Sao Carlos, 13566-970, Brazil.
  • Dos Santos GR; Sao Carlos Institute of Chemistry, University of Sao Paulo (USP), Sao Carlos, 13566-590, Brazil.
  • Pinto CAL; Department of Morphology and Pathology, Jundiai Medical School, Jundiai, 13202-550, Brazil.
  • Pelegrine AA; Department of Morphology and Pathology, Jundiai Medical School, Jundiai, 13202-550, Brazil.
  • Teixeira ML; Department of Implant Dentistry, Faculdade Sao Leopoldo Mandic, Campinas, 13045-755, Brazil.
  • Buchaim DV; Prosthodontics Department, Faculdade Sao Leopoldo Mandic, Campinas, 13045-755, Brazil.
  • Nazari FM; Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia, 17525-902, Brazil.
  • Buchaim RL; Teaching and Research Coordination of the Medical School, University Center of Adamantina (UNIFAI), Adamantina, 17800-000, Brazil.
  • Sugano GT; Department of Morphology and Pathology, Jundiai Medical School, Jundiai, 13202-550, Brazil.
  • da Cunha MR; Department of Biological Sciences, Bauru School of Dentistry (FOB), University of Sao Paulo (USP), Bauru, 17012-901, Brazil.
Sci Rep ; 13(1): 28, 2023 01 02.
Article en En | MEDLINE | ID: mdl-36593236
Lesions with bone loss may require autologous grafts, which are considered the gold standard; however, natural or synthetic biomaterials are alternatives that can be used in clinical situations that require support for bone neoformation. Collagen and hydroxyapatite have been used for bone repair based on the concept of biomimetics, which can be combined with chitosan, forming a scaffold for cell adhesion and growth. However, osteoporosis caused by gonadal hormone deficiency can thus compromise the expected results of the osseointegration of scaffolds. The aim of this study was to investigate the osteoregenerative capacity of collagen (Co)/chitosan (Ch)/hydroxyapatite (Ha) scaffolds in rats with hormone deficiency caused by experimental bilateral ovariectomy. Forty-two rats were divided into non-ovariectomized (NO) and ovariectomized (O) groups, divided into three subgroups: control (empty defect) and two subgroups receiving collagen/chitosan/hydroxyapatite scaffolds prepared using different methods of hydroxyapatite incorporation, in situ (CoChHa1) and ex situ (CoChHa2). The defect areas were submitted to macroscopic, radiological, and histomorphometric analysis. No inflammatory processes were found in the tibial defect area that would indicate immune rejection of the scaffolds, thus confirming the biocompatibility of the biomaterials. Bone formation starting from the margins of the bone defect were observed in all rats, with a greater volume in the NO groups, particularly the group receiving CoChHa2. Less bone formation was found in the O subgroups when compared to the NO. In conclusion, collagen/chitosan/hydroxyapatite scaffolds stimulate bone growth in vivo but abnormal conditions of bone fragility caused by gonadal hormone deficiency may have delayed the bone repair process.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Durapatita / Quitosano Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Durapatita / Quitosano Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido