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Infertile human endometrial organoid apical protein secretions are dysregulated and impair trophoblast progenitor cell adhesion.
Zhou, Wei; Barton, Siena; Cui, Jinwei; Santos, Leilani L; Yang, Guannan; Stern, Catharyn; Kieu, Violet; Teh, Wan Tinn; Ang, Catarina; Lucky, Tarana; Sgroi, Joseph; Ye, Louie; Dimitriadis, Evdokia.
Afiliación
  • Zhou W; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
  • Barton S; Gynaecology Research Centre, Royal Women's Hospital, Parkville, VIC,  Australia.
  • Cui J; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
  • Santos LL; Gynaecology Research Centre, Royal Women's Hospital, Parkville, VIC,  Australia.
  • Yang G; Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW,  Australia.
  • Stern C; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
  • Kieu V; Gynaecology Research Centre, Royal Women's Hospital, Parkville, VIC,  Australia.
  • Teh WT; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
  • Ang C; Gynaecology Research Centre, Royal Women's Hospital, Parkville, VIC,  Australia.
  • Lucky T; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
  • Sgroi J; The Royal Women's Hospital, Parkville, VIC,  Australia.
  • Ye L; Melbourne IVF, Melbourne, VIC, Australia.
  • Dimitriadis E; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC,  Australia.
Front Endocrinol (Lausanne) ; 13: 1067648, 2022.
Article en En | MEDLINE | ID: mdl-36589798
Introduction: Embryo implantation failure leads to infertility. As an important approach to regulate implantation, endometrial epithelial cells produce and secrete factors apically into the uterine cavity in the receptive phase to prepare the initial blastocyst adhesion and implantation. Organoids were recently developed from human endometrial epithelium with similar apical-basal polarity compared to endometrial gland making it an ideal model to study endometrial epithelial secretions. Methods: Endometrial organoids were established using endometrial biopsies from women with primary infertility and normal fertility. Fertile and infertile organoids were treated with hormones to model receptive phase of the endometrial epithelium and intra-organoid fluid (IOF) was collected to compare the apical protein secretion profile and function on trophoblast cell adhesion. Results: Our data show that infertile organoids were dysregulated in their response to estrogen and progesterone treatment. Proteomic analysis of organoid apical secretions identified 150 dysregulated proteins between fertile and infertile groups (>1.5-fold change). Trophoblast progenitor spheroids (blastocyst surrogates) treated with infertile organoid apical secretions significantly compromised their adhesion to organoid epithelial cell monolayers compared to fertile group (P < 0.0001). Discussion: This study revealed that endometrial organoid apical secretions alter trophoblast cell adhesiveness relative to fertility status of women. It paves the way to determine the molecular mechanisms by which endometrial epithelial apical released factors regulate blastocyst initial attachment and implantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / Infertilidad Femenina Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Front Endocrinol (Lausanne) Año: 2022 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / Infertilidad Femenina Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Front Endocrinol (Lausanne) Año: 2022 Tipo del documento: Article Pais de publicación: Suiza