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Noninvasive prenatal diagnosis targeting fetal nucleated red blood cells.
Chen, Yanyu; Wu, Zhuhao; Sutlive, Joseph; Wu, Ke; Mao, Lu; Nie, Jiabao; Zhao, Xing-Zhong; Guo, Feng; Chen, Zi; Huang, Qinqin.
Afiliación
  • Chen Y; The Research and Application Center of Precision Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China.
  • Wu Z; School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
  • Sutlive J; Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, 47405, United States.
  • Wu K; Division of Thoracic and Cardiac Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
  • Mao L; School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
  • Nie J; The Research and Application Center of Precision Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China.
  • Zhao XZ; Division of Thoracic and Cardiac Surgery, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
  • Guo F; Department of Biological Sciences, Northeastern University, Boston, MA, 02115, United States.
  • Chen Z; School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
  • Huang Q; Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, 47405, United States. fengguo@iu.edu.
J Nanobiotechnology ; 20(1): 546, 2022 Dec 30.
Article en En | MEDLINE | ID: mdl-36585678
Noninvasive prenatal diagnosis (NIPD) aims to detect fetal-related genetic disorders before birth by detecting markers in the peripheral blood of pregnant women, holding the potential in reducing the risk of fetal birth defects. Fetal-nucleated red blood cells (fNRBCs) can be used as biomarkers for NIPD, given their remarkable nature of carrying the entire genetic information of the fetus. Here, we review recent advances in NIPD technologies based on the isolation and analysis of fNRBCs. Conventional cell separation methods rely primarily on physical properties and surface antigens of fNRBCs, such as density gradient centrifugation, fluorescence-activated cell sorting, and magnetic-activated cell sorting. Due to the limitations of sensitivity and purity in Conventional methods, separation techniques based on micro-/nanomaterials have been developed as novel methods for isolating and enriching fNRBCs. We also discuss emerging methods based on microfluidic chips and nanostructured substrates for static and dynamic isolation of fNRBCs. Additionally, we introduce the identification techniques of fNRBCs and address the potential clinical diagnostic values of fNRBCs. Finally, we highlight the challenges and the future directions of fNRBCs as treatment guidelines in NIPD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Prenatales no Invasivas Tipo de estudio: Diagnostic_studies / Guideline Límite: Female / Humans / Pregnancy Idioma: En Revista: J Nanobiotechnology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Prenatales no Invasivas Tipo de estudio: Diagnostic_studies / Guideline Límite: Female / Humans / Pregnancy Idioma: En Revista: J Nanobiotechnology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido