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Characterization of proteogenomic signatures of differentiation of CD4+ T cell subsets.
Kanno, Toshio; Konno, Ryo; Miyako, Keisuke; Nakajima, Takahiro; Yokoyama, Satoru; Sasamoto, Shigemi; Asou, Hikari K; Ohzeki, Junichiro; Kawashima, Yusuke; Hasegawa, Yoshinori; Ohara, Osamu; Endo, Yusuke.
Afiliación
  • Kanno T; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Konno R; Department of Applied Genomics Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Miyako K; Department of Applied Genomics Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Nakajima T; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Yokoyama S; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Sasamoto S; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Asou HK; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Ohzeki J; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Kawashima Y; Department of Applied Genomics Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Hasegawa Y; Department of Applied Genomics Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Ohara O; Department of Applied Genomics Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Endo Y; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
DNA Res ; 30(1)2023 Feb 01.
Article en En | MEDLINE | ID: mdl-36579714
Functionally distinct CD4+ helper T (Th) cell subsets, including Th1, Th2, Th17, and regulatory T cells (Treg), play a pivotal role in the regulation of acquired immunity. Although the key proteins involved in the regulation of Th cell differentiation have already been identified how the proteogenomic landscape changes during the Th cell activation remains unclear. To address this issue, we characterized proteogenomic signatures of differentiation to each Th cell subsets by RNA sequencing and liquid chromatography-assisted mass spectrometry, which enabled us to simultaneously quantify more than 10,000 protein-coding transcripts and 8,000 proteins in a single-shot. The results indicated that T cell receptor activation affected almost half of the transcript and protein levels in a low correlative and gene-specific manner, and specific cytokine treatments modified the transcript and protein profiles in a manner specific to each Th cell subsets: Th17 and Tregs particularly exhibited unique proteogenomic signatures compared to other Th cell subsets. Interestingly, the in-depth proteome data revealed that mRNA profiles alone were not enough to delineate functional changes during Th cell activation, suggesting that the proteogenomic dataset obtained in this study serves as a unique and indispensable data resource for understanding the comprehensive molecular mechanisms underlying effector Th cell differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Proteogenómica Idioma: En Revista: DNA Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Proteogenómica Idioma: En Revista: DNA Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido