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Characterization of two novel ammonia transporters, HIAT1α and HIAT1ß, in the American Horseshoe Crab, Limulus polyphemus.
Sachs, Maria; Quijada-Rodriguez, Alex R; Hans, Stephanie; Weihrauch, Dirk.
Afiliación
  • Sachs M; University of Manitoba, Department of Biological Sciences, 66 Chancellors Circle, Winnipeg, MB R3T 2N2, Canada.
  • Quijada-Rodriguez AR; University of Manitoba, Department of Biological Sciences, 66 Chancellors Circle, Winnipeg, MB R3T 2N2, Canada.
  • Hans S; University of Manitoba, Department of Biological Sciences, 66 Chancellors Circle, Winnipeg, MB R3T 2N2, Canada.
  • Weihrauch D; University of Manitoba, Department of Biological Sciences, 66 Chancellors Circle, Winnipeg, MB R3T 2N2, Canada. Electronic address: Dirk.Weihrauch@umanitoba.ca.
Comp Biochem Physiol A Mol Integr Physiol ; 278: 111365, 2023 04.
Article en En | MEDLINE | ID: mdl-36577451
The American horseshoe crab, Limulus polyphemus, excretes nitrogenous waste in the form of toxic ammonia across their book gills. The mechanism of this branchial excretion is yet unknown. In the current study, two isoforms of a novel ammonia transporter, LpHIAT1α and LpHIAT1ß, have been identified in L. polyphemus. Both isoforms have 12 predicted transmembrane regions and share 82.7% of amino acid identity to each other, and 77-86% amino acid homology to HIAT1 found in fish and crustaceans. In L. polyphemus, both isoforms were expressed in the gills, coxal glands, and brain. Slightly higher mRNA expression levels of LpHIAT1α were observed in the peripheral mitochondria-poor region of the gill (PMPA), central mitochondria-rich region of the gill (CMRA), and brain compared to the LpHIAT1ß isoform. A functional expression analysis of LpHIAT1α and LpHIAT1ß in Xenopus laevis oocytes resulted in a significantly lower uptake of the radiolabeled ammonia analogue 3H-methylamine when compared to controls, indicating an ammonia excretory function of the proteins. Exposure to elevated environmental ammonia (HEA, 1 mmol l-1 NH4Cl) caused an increase in mRNA expression of LpHIAT1ß in the ion-conductive ventral gill half. High mRNA expression of both isoforms in the brain, and the observation that LpHIAT1α and LpHIAT1ß likely mediate cellular ammonia excretion, suggests that these highly conserved ammonia transporters have an important housekeeping function in cellular ammonia elimination.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Amoníaco / Cangrejos Herradura Límite: Animals Idioma: En Revista: Comp Biochem Physiol A Mol Integr Physiol Asunto de la revista: BIOLOGIA MOLECULAR / FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Amoníaco / Cangrejos Herradura Límite: Animals Idioma: En Revista: Comp Biochem Physiol A Mol Integr Physiol Asunto de la revista: BIOLOGIA MOLECULAR / FISIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos