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Photoelectrochemical imaging of single cardiomyocytes and monitoring of their action potentials through contact force manipulation of organoids.
Jacques, Rachel; Zhou, Bo; Marhuenda, Emilie; Gorecki, Jon; Das, Anirban; Iskratsch, Thomas; Krause, Steffi.
Afiliación
  • Jacques R; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK.
  • Zhou B; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. Electronic address: bo.zhou@qmul.ac.uk.
  • Marhuenda E; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK.
  • Gorecki J; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK.
  • Das A; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK.
  • Iskratsch T; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. Electronic address: t.iskratsch@qmul.ac.uk.
  • Krause S; School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, UK. Electronic address: s.krause@qmul.ac.uk.
Biosens Bioelectron ; 223: 115024, 2023 Mar 01.
Article en En | MEDLINE | ID: mdl-36577176
Accurate monitoring of cardiomyocyte action potentials (APs) is essential to understand disease propagation and for trials of novel therapeutics. Patch clamp techniques offer 'gold standard' measurements in this field, but are notoriously difficult to operate and only provide measurements of a single cell. Here we propose photoelectrochemical imaging (PEI) with light-addressable potentiometric sensors (LAPS) in conjunction with a setup for controlling the contact force between the cardiomyocyte organoids and the sensor surface for measuring APs with high sensitivity. The method was validated through measuring the responses to drugs, and the results successfully visualized the expected electrophysiological changes to the APs. PEI allows for several cells to be monitored simultaneously, opening further research to the electrophysiological interactions of adjoining cells. This method expands the applications of PEI to three-dimensional geometries and provides the fields of stem cell research, drug trials and heart disease modelling with an invaluable tool to further investigate the role of APs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Miocitos Cardíacos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Miocitos Cardíacos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido