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Unusual aldehyde reductase activity for the production of full-length fatty alcohol by cyanobacterial aldehyde deformylating oxygenase.
Treesukkasem, Nidar; Buttranon, Supacha; Intasian, Pattarawan; Jaroensuk, Juthamas; Maenpuen, Somchart; Sucharitakul, Jeerus; Lawan, Narin; Chaiyen, Pimchai; Wongnate, Thanyaporn.
Afiliación
  • Treesukkasem N; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
  • Buttranon S; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
  • Intasian P; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
  • Jaroensuk J; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
  • Maenpuen S; Department of Biochemistry, Faculty of Science, Burapha University, Chonburi, 20131, Thailand.
  • Sucharitakul J; Department of Biochemistry and Skeletal Disorders Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10300, Thailand.
  • Lawan N; Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
  • Chaiyen P; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
  • Wongnate T; School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand. Electronic address: thanyaporn.w@vistec.ac.th.
Arch Biochem Biophys ; 734: 109498, 2023 01 15.
Article en En | MEDLINE | ID: mdl-36572346
Aldehyde-deformylating oxygenase (ADO) is a non-heme di-iron enzyme that catalyzes the deformylation of aldehydes to generate alkanes/alkenes. In this study, we report for the first time that under anaerobic or limited oxygen conditions, Prochlorococcus marinus (PmADO) can generate full-length fatty alcohols from fatty aldehydes without eliminating a carbon unit. In contrast to ADO's native activity, which requires electrons from the Fd/FNR electron transfer complex, ADO's aldehyde reduction activity requires only NAD(P)H. Our results demonstrated that the yield of alcohol products could be affected by oxygen concentration and the type of aldehyde. Under strictly anaerobic conditions, yields of octanol were up to 31%. Moreover, metal cofactors are not involved in the aldehyde reductase activity of PmADO because the yields of alcohols obtained from apoenzyme and holoenzyme treated with various metals were similar under anaerobic conditions. In addition, PmADO prefers medium-chain aldehydes, specifically octanal (kcat/Km around 15 × 10-3 µM-1min-1). The findings herein highlight a new activity of PmADO, which may be applied as a biocatalyst for the industrial synthesis of fatty alcohols.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aldehído Reductasa / Cianobacterias Idioma: En Revista: Arch Biochem Biophys Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aldehído Reductasa / Cianobacterias Idioma: En Revista: Arch Biochem Biophys Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos