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A nanobody against the VWF A3 domain detects ADAMTS13-induced proteolysis in congenital and acquired VWD.
Kizlik-Masson, Claire; Peyron, Ivan; Gangnard, Stéphane; Le Goff, Gaelle; Lenoir, Solen M; Damodaran, Sandra; Clavel, Marie; Roullet, Stéphanie; Regnault, Véronique; Rauch, Antoine; Vincent, Flavien; Jeanpierre, Emmanuelle; Dupont, Annabelle; Ternisien, Catherine; Donnet, Thibault; Christophe, Olivier D; van Belle, Eric; Denis, Cécile V; Casari, Caterina; Susen, Sophie; Lenting, Peter J.
Afiliación
  • Kizlik-Masson C; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Peyron I; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Gangnard S; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Le Goff G; Diagnostica Stago, Unités de recherche & développement, Gennevilliers, France.
  • Lenoir SM; Diagnostica Stago, Unités de recherche & développement, Gennevilliers, France.
  • Damodaran S; Diagnostica Stago, Unités de recherche & développement, Gennevilliers, France.
  • Clavel M; Inovarion, Paris, France.
  • Roullet S; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Regnault V; Université de Lorraine, Laboratory for Acute and Chronic Cardiovascular Deficiency (DCAC), Institut National de la Santé et de la Recherche Médicale Unité 1116, Nancy, France.
  • Rauch A; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Vincent F; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Jeanpierre E; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Dupont A; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Ternisien C; French Reference Center for von Willebrand Disease (CRMW), Lille, France.
  • Donnet T; Diagnostica Stago, Unités de recherche & développement, Gennevilliers, France.
  • Christophe OD; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • van Belle E; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Denis CV; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Casari C; Laboratory for Hemostasis, Inflammation & Thrombosis, Unité Mixte de Recherche 1176, Institut National de la Santé et de la Recherche Médicale, Université Paris-Saclay, Le Kremlin-Bicêtre, France.
  • Susen S; Université de Lille, Centre Hospitalier Universitaire Lille, Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale Unité 1011, Lille, France.
  • Lenting PJ; French Reference Center for von Willebrand Disease (CRMW), Lille, France.
Blood ; 141(12): 1457-1468, 2023 03 23.
Article en En | MEDLINE | ID: mdl-36564031
von Willebrand factor (VWF) is a multimeric protein, the size of which is regulated via ADAMTS13-mediated proteolysis within the A2 domain. We aimed to isolate nanobodies distinguishing between proteolyzed and non-proteolyzed VWF, leading to the identification of a nanobody (designated KB-VWF-D3.1) targeting the A3 domain, the epitope of which overlaps the collagen-binding site. Although KB-VWF-D3.1 binds with similar efficiency to dimeric and multimeric derivatives of VWF, binding to VWF was lost upon proteolysis by ADAMTS13, suggesting that proteolysis in the A2 domain modulates exposure of its epitope in the A3 domain. We therefore used KB-VWF-D3.1 to monitor VWF degradation in plasma samples. Spiking experiments showed that a loss of 10% intact VWF could be detected using this nanobody. By comparing plasma from volunteers to that from congenital von Willebrand disease (VWD) patients, intact-VWF levels were significantly reduced for all VWD types, and most severely in VWD type 2A-group 2, in which mutations promote ADAMTS13-mediated proteolysis. Unexpectedly, we also observed increased proteolysis in some patients with VWD type 1 and VWD type 2M. A significant correlation (r = 0.51, P < .0001) between the relative amount of high-molecular weight multimers and levels of intact VWF was observed. Reduced levels of intact VWF were further found in plasmas from patients with severe aortic stenosis and patients receiving mechanical circulatory support. KB-VWF-D3.1 is thus a nanobody that detects changes in the exposure of its epitope within the collagen-binding site of the A3 domain. In view of its unique characteristics, it has the potential to be used as a diagnostic tool to investigate whether a loss of larger multimers is due to ADAMTS13-mediated proteolysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de von Willebrand / Enfermedad de von Willebrand Tipo 2 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Blood Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de von Willebrand / Enfermedad de von Willebrand Tipo 2 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Blood Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos