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Efficient decolorization and detoxification of triarylmethane and azo dyes by porous-cross-linked enzyme aggregates of Pleurotus ostreatus laccase.
George, Jenet; Rajendran, Devi Sri; Senthil Kumar, P; Sonai Anand, Srinidhi; Vinoth Kumar, Vaidyanathan; Rangasamy, Gayathri.
Afiliación
  • George J; Integrated Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203, India.
  • Rajendran DS; Integrated Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203, India.
  • Senthil Kumar P; Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam 603 110, Tamil Nadu, India; Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam 603 110, Tamil Nadu, India; School of Engineering, Lebanese Americ
  • Sonai Anand S; Integrated Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203, India.
  • Vinoth Kumar V; Integrated Bioprocess Laboratory, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai 603203, India. Electronic address: vinothkv@srmist.edu.in.
  • Rangasamy G; School of Engineering, Lebanese American University, Byblos, Lebanon; University Centre for Research and Development & Department of Civil Engineering, Chandigarh University, Gharuan, Mohali, Punjab 140413, India.
Chemosphere ; 313: 137612, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36563730
In this preset study, porous-cross-linked enzyme aggregates (CLEAs) of Pleurotus ostreatus laccase were utilized for the spontaneous decolorization and detoxification of triarylmethane and azo dyes, reactive blue 2 (RB) and malachite green (MG). The specific surface area and pore radius of the porous-CLEAs are 136.3 m2/g and 19.47 Ao, and the higher specific surface indicated greater biocatalytic efficiency, as increased mass transfer and dye interaction with the CLEAs laccase. CLEAs laccase decolorized 500 ppm of MG and RB with 98.12-58.33% efficiency after 120 min, at pH 5.0 and 50°C, without a mediator. Furthermore, the biotransformation of the MG and RB with immobilized laccase was confirmed with the help of UV-visible spectroscopy, high-performance liquid chromatography, and Fourier transform infrared spectroscopy. The reusability potential of CLEAs was assessed in batch mode for 10 cycles of dye decolorization. The decolorization activities for the immobilized laccase were 89% and 12% at the 6th cycle for MG and RB, respectively. This immobilized enzyme could effectively remove dyes from aqueous solution, and demonstrated significant detoxification in experimental plants (Triticum aestivum and Phaseolus mungo) and plant growth-promoting rhizobacteria (Azospirillum brasilense, Bacillus megaterium, Rhizobium leguminosarum, Bacillus subtilis, and Pseudomonas fluorescens). In conclusion, porous CLEAs laccase could be useful as a potential bioremediation tool for the detoxification and decolorization of dyeing wastewater in future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pleurotus / Lacasa Idioma: En Revista: Chemosphere Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pleurotus / Lacasa Idioma: En Revista: Chemosphere Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido