Radioimmunoassay of 5-hydroxymethyl-2'-deoxyuridine.
J Immunol Methods
; 103(1): 41-5, 1987 Oct 23.
Article
en En
| MEDLINE
| ID: mdl-3655385
5-Hydroxymethyl-2'-deoxyuridine is an antileukemic thymidine analogue. It is also a well known thymidine-derivative in DNA exposed to ionizing irradiation. We report the production and characterization of specific rabbit anti-5HmdUrd antisera. The antisera were used for the radioimmunological measurement of 5HmdUrd. The radioimmunoassay was capable of quantitating 2 pmol of 5 HmdUrd per tube corresponding to 0.2 mumol/l in a 10 microliter plasma sample. A good correlation between the results obtained with the radioimmunoassay and HPLC was demonstrated when the methods were applied to the measurement of plasma levels of 5HmdUrd in mice receiving experimental chemotherapy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Timidina
/
Radioinmunoensayo
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
1987
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Países Bajos