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Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.
Pagliari, Francesca; Sogne, Elisa; Panella, Davide; Perozziello, Gerardo; Liberale, Carlo; Das, Gobind; Turdo, Alice; Di Franco, Simone; Seco, Joao; Falqui, Andrea; Gratteri, Santo; Pujia, Arturo; Di Fabrizio, Enzo; Candeloro, Patrizio; Tirinato, Luca.
Afiliación
  • Pagliari F; Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
  • Sogne E; Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Panella D; Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Perozziello G; PoliFAB, Polytechnic of Milan, Via Giuseppe Colombo, 81, 20133 Milan, Italy.
  • Liberale C; Nanotechnology Research Center, Department of Experimental and Clinical Medicine, University of Magna Graecia, 88100 Catanzaro, Italy.
  • Das G; Nanotechnology Research Center, Department of Experimental and Clinical Medicine, University of Magna Graecia, 88100 Catanzaro, Italy.
  • Turdo A; Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Di Franco S; Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
  • Seco J; Department of Physics, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates.
  • Falqui A; Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties (PROMISE), University of Palermo, 90127 Palermo, Italy.
  • Gratteri S; Department of Surgical, Oncological and Stomatological Sciences (DICHIRONS), University of Palermo, 90127 Palermo, Italy.
  • Pujia A; Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
  • Di Fabrizio E; Department of Physics and Astronomy, Heidelberg University, 69120 Heidelberg, Germany.
  • Candeloro P; Department of Physics "Aldo Pontremoli", University of Milan, Via Celoria 16, 20133 Milan, Italy.
  • Tirinato L; Institute of Research for Food Safety & Health IRC-FSH, University Magna Graecia, 88100 Catanzaro, Italy.
Biosensors (Basel) ; 12(12)2022 Dec 01.
Article en En | MEDLINE | ID: mdl-36551069
Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light-electron microscopy and by correlative Raman imaging-scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gotas Lipídicas / Melanoma Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gotas Lipídicas / Melanoma Límite: Humans Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza