Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase-mediated cassette exchange hybrid system.

Ghanbari, Samaneh; Bayat, Elham; Azizi, Masoumeh; Fard-Esfahani, Pezhman; Modarressi, Mohammad Hossein; Davami, Fatemeh

Ghanbari, Samaneh; Bayat, Elham; Azizi, Masoumeh; Fard-Esfahani, Pezhman; Modarressi, Mohammad Hossein; Davami, Fatemeh.

Afiliación

Ghanbari S; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Bayat E; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Azizi M; Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Fard-Esfahani P; Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Modarressi MH; Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Davami F; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. f.davami@gmail.com.

Appl Microbiol Biotechnol ; 107(2-3): 769-783, 2023 Feb.

Article en En | MEDLINE | ID: mdl-36536089

Asunto(s)

Genoma; Recombinasas; Cricetinae; Animales; Humanos; Células CHO; Cricetulus; Recombinasas/genética; Transgenes

Palabras clave

CHO cell line development (CLD); CRIS-PITCh; Microhomology-mediated end joining (MMEJ); Random integration (RI); Recombinase-mediated cassette exchange (RMCE); Targeted integration (TI)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma / Recombinasas Límite: Animals / Humans Idioma: En Revista: Appl Microbiol Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Alemania

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