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Metabolism of Scenedesmus obliquus cultivated with raw plant substrates.
Schambach, Jenna Y; Kruse, Colin P S; Kitin, Peter; Mays, Wittney; Hunt, Christopher G; Starkenburg, Shawn R; Barry, Amanda N.
Afiliación
  • Schambach JY; Molecular and Microbiology Department, Sandia National Laboratories, Albuquerque, NM, United States.
  • Kruse CPS; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States.
  • Kitin P; Forest Products Laboratory, Forest Biopolymer Science and Engineering, U.S. Forest Service, Madison, WI, United States.
  • Mays W; Computational Biology and Biophysics Department, Sandia National Laboratories, Albuquerque, NM, United States.
  • Hunt CG; Forest Products Laboratory, Forest Biopolymer Science and Engineering, U.S. Forest Service, Madison, WI, United States.
  • Starkenburg SR; Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United States.
  • Barry AN; Molecular and Microbiology Department, Sandia National Laboratories, Albuquerque, NM, United States.
Front Plant Sci ; 13: 992702, 2022.
Article en En | MEDLINE | ID: mdl-36531386
The potential benefits of adding raw, non-food, lignocellulosic plant material as a carbon source for mixotrophic growth of microalgae have previously been demonstrated. This approach has advantages over using traditional carbon sources like glucose or acetate due to wide-spread plant biomass availability and substrate recalcitrance to bacterial contamination. Here, we report the overall growth characteristics and explore the metabolic patterns of Scenedesmus obliquus cultured in the presence raw plant substrate. An initial screen of plant substrate candidates showed an increase in specific growth rate and biomass accumulation when S. obliquus was cultured in the presence of switchgrass or yard waste compared to media alone. We observed a near doubling of microalgal dry weight when S. obliquus was grown with 0.2% (w/v) switchgrass under ambient CO2. Scanning electron microscopy (SEM) of corn stem after S. obliquus cultivation exhibited substantial phloem degradation. Transcriptomic analyses of S. obliquus during mid- and late-log phase growth revealed a dynamic metabolic landscape within many KEGG pathways. Notably, differential expression was observed for several potential glycosyl hydrolases. We also investigated the influence of switchgrass on the growth of S. obliquus at 50 L volume in mini raceway ponds to determine the scalability of this approach.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza