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Single-photon smFRET. III. Application to pulsed illumination.
Safar, Matthew; Saurabh, Ayush; Sarkar, Bidyut; Fazel, Mohamadreza; Ishii, Kunihiko; Tahara, Tahei; Sgouralis, Ioannis; Pressé, Steve.
Afiliación
  • Safar M; Center for Biological Physics, Arizona State University, Tempe, Arizona.
  • Saurabh A; Department of Mathematics and Statistical Science, Arizona State University, Tempe, Arizona.
  • Sarkar B; Center for Biological Physics, Arizona State University, Tempe, Arizona.
  • Fazel M; Department of Physics, Arizona State University, Tempe, Arizona.
  • Ishii K; Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, Japan.
  • Tahara T; Ultrafast Spectroscopy Research Team, RIKEN Center for Advanced Photonics (RAP), 2-1 Hirosawa, Wako, Saitama, Japan.
  • Sgouralis I; Center for Biological Physics, Arizona State University, Tempe, Arizona.
  • Pressé S; Department of Physics, Arizona State University, Tempe, Arizona.
Biophys Rep (N Y) ; 2(4): 100088, 2022 Dec 14.
Article en En | MEDLINE | ID: mdl-36530182
Förster resonance energy transfer (FRET) using pulsed illumination has been pivotal in leveraging lifetime information in FRET analysis. However, there remain major challenges in quantitative single-photon, single-molecule FRET (smFRET) data analysis under pulsed illumination including 1) simultaneously deducing kinetics and number of system states; 2) providing uncertainties over estimates, particularly uncertainty over the number of system states; and 3) taking into account detector noise sources such as cross talk and the instrument response function contributing to uncertainty; in addition to 4) other experimental noise sources such as background. Here, we implement the Bayesian nonparametric framework described in the first companion article that addresses all aforementioned issues in smFRET data analysis specialized for the case of pulsed illumination. Furthermore, we apply our method to both synthetic as well as experimental data acquired using Holliday junctions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biophys Rep (N Y) Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biophys Rep (N Y) Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos