Binding of active Ras and its mutants to the Ras binding domain of PI-3-kinase: A quantitative approach to K<sub>D</sub> measurements.

Fleming, Ian R; Hannan, Jonathan P; Swisher, George Hayden; Tesdahl, Corey D; Martyr, Justin G; Cordaro, Nicholas J; Erbse, Annette H; Falke, Joseph J

Binding of active Ras and its mutants to the Ras binding domain of PI-3-kinase: A quantitative approach to K_D measurements.

Fleming, Ian R; Hannan, Jonathan P; Swisher, George Hayden; Tesdahl, Corey D; Martyr, Justin G; Cordaro, Nicholas J; Erbse, Annette H; Falke, Joseph J.

Afiliación

Fleming IR; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Hannan JP; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Swisher GH; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Tesdahl CD; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Martyr JG; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Cordaro NJ; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Erbse AH; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA.
Falke JJ; Molecular Biophysics Program and Department of Biochemistry, University of Colorado, Boulder, CO, 80309-0596, USA. Electronic address: falke@colorado.edu.

Anal Biochem ; 663: 115019, 2023 02 15.

Article en En | MEDLINE | ID: mdl-36526022

Asunto(s)

Fosfatidilinositol 3-Quinasas; Proteínas ras; Fosfatidilinositol 3-Quinasas/genética; Fosfatidilinositol 3-Quinasas/química; Fosfatidilinositol 3-Quinasas/metabolismo; Proteínas ras/química; Unión Proteica; Guanosina Trifosfato/metabolismo; Fosfatidilinositoles

Palabras clave

GMPPN; GMPPNP; GTP; HRas GTPase; Protein-nucleotide complex; Ras small G protein

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas ras / Fosfatidilinositol 3-Quinasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Anal Biochem Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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