Point-of-care assay platform for uric acid, urea, and triglycerides with a microfluidic paper device (µPAD) controlled by stimulus-sensitive valves.
Talanta
; 254: 124189, 2023 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-36525868
A microfluidic biosensor paper-based analytical device, or µPAD, was developed as a point-of-care assay platform for the simultaneous determination of three clinically important markers: uric acid, urea, and triglycerides. To achieve a simultaneous determination, thermosensitive valves based on N-isopropylacrylamide polymers controlled the flow of fluid in the µPAD. The evaluation of the analytical characteristics of the µPAD was done using a photographic camera at room temperature. The detection limits were 4.5·10-5, 2.5·10-3, and 1.5·10-3 mg mL-1, for uric acid, urea, and triglycerides, respectively, and the precision expressed as relative standard deviation (% RSD) 3.2, 1.2, and 1.6%. The obtained µPAD devices were validated with real human plasma samples, demonstrating high accuracy and precision. Multiple analyte point-of-care tests such as those developed here have excellent characteristics, being easy to manufacture, cost-effective, easy to use, and highly sensitive.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Úrico
/
Técnicas Analíticas Microfluídicas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2023
Tipo del documento:
Article
Pais de publicación:
Países Bajos