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Single-Cell and Spatial Transcriptomics Decodes Wharton's Jelly-Derived Mesenchymal Stem Cells Heterogeneity and a Subpopulation with Wound Repair Signatures.
Chen, Penghong; Tang, Shijie; Li, Ming; Wang, Dezhi; Chen, Caixiang; Qiu, Yiqun; Fang, Zhuoqun; Zhang, Haoruo; Gao, Hangqi; Weng, Haiyan; Hu, Kailun; Lin, Jian; Lin, Qingxia; Tan, Yi; Li, Shirong; Chen, Jinghua; Chen, Liangwan; Chen, Xiaosong.
Afiliación
  • Chen P; Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
  • Tang S; Department of Plastic Surgery and Regenerative Medicine Institute, Fujian Medical University, Fuzhou, 350001, China.
  • Li M; Engineering Research Center of Tissue and Organ Regeneration, Fujian Province University, Fuzhou, 350001, China.
  • Wang D; Department of Stem Cell Research Institute, Fujian Medical University, Fuzhou, 350004, China.
  • Chen C; Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
  • Qiu Y; Department of Plastic Surgery and Regenerative Medicine Institute, Fujian Medical University, Fuzhou, 350001, China.
  • Fang Z; Engineering Research Center of Tissue and Organ Regeneration, Fujian Province University, Fuzhou, 350001, China.
  • Zhang H; Department of Stem Cell Research Institute, Fujian Medical University, Fuzhou, 350004, China.
  • Gao H; Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
  • Weng H; Department of Plastic Surgery and Regenerative Medicine Institute, Fujian Medical University, Fuzhou, 350001, China.
  • Hu K; Engineering Research Center of Tissue and Organ Regeneration, Fujian Province University, Fuzhou, 350001, China.
  • Lin J; Department of Stem Cell Research Institute, Fujian Medical University, Fuzhou, 350004, China.
  • Lin Q; Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
  • Tan Y; Department of Plastic Surgery and Regenerative Medicine Institute, Fujian Medical University, Fuzhou, 350001, China.
  • Li S; Engineering Research Center of Tissue and Organ Regeneration, Fujian Province University, Fuzhou, 350001, China.
  • Chen J; Department of Stem Cell Research Institute, Fujian Medical University, Fuzhou, 350004, China.
  • Chen L; Department of Plastic Surgery and Regenerative Medicine, Fujian Medical University Union Hospital, Fuzhou, 350001, China.
  • Chen X; Department of Plastic Surgery and Regenerative Medicine Institute, Fujian Medical University, Fuzhou, 350001, China.
Adv Sci (Weinh) ; 10(4): e2204786, 2023 02.
Article en En | MEDLINE | ID: mdl-36504438
The highly heterogeneous characteristics of Wharton's jelly mesenchymal stem cells (WJ-MSCs) may be responsible for the poor clinical outcomes and poor reproducibility of treatments based on WJ-MSCs. Exploration of WJ-MSC heterogeneity with multimodal single-cell technologies will aid in establishing accurate MSC subtyping and developing screening protocols for dominant functional subpopulations. Here, the characteristics of WJ-MSCs are systematically analyzed by single cell and spatial transcriptome sequencing. Single-cell transcriptomics analysis identifies four WJ-MSC subpopulations, namely proliferative_MSCs, niche-supporting_MSCs, metabolism-related_MSCs and biofunctional-type_MSCs. Furthermore, the transcriptome, cellular heterogeneity, and cell-state trajectories of these subpopulations are characterized. Intriguingly, the biofunctional-type MSCs (marked by S100A9, CD29, and CD142) selected in this study exhibit promising wound repair properties in vitro and in vivo. Finally, by integrating omics data, it has been found that the S100A9+ CD29+ CD142+ subpopulation is more enriched in the fetal segment of the umbilical cord, suggesting that this subpopulation deriving from the fetal segment may have potential for developing into an ideal therapeutic agent for wound healing. Overall, the presented study comprehensively maps the heterogeneity of WJ-MSCs and provides an essential resource for future development of WJ-MSC-based drugs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gelatina de Wharton / Células Madre Mesenquimatosas Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gelatina de Wharton / Células Madre Mesenquimatosas Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: Adv Sci (Weinh) Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania