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Gene amplification mutations originate prior to selective stress in Acinetobacter baylyi.
Herrmann, Jennifer A; Koprowska, Agata; Winters, Tesa J; Villanueva, Nancy; Nikityuk, Victoria D; Pek, Feini; Reis, Elizabeth M; Dominguez, Constancia Z; Davis, Daniel; McPherson, Eric; Rocco, Staci R; Recendez, Cynthia; Difuntorum, Shyla M; Faeth, Kelly; Lopez, Mario D; Awwad, Habeeba M; Ghobashy, Rola A; Cappiello, Lauren; Neidle, Ellen L; Quiñones-Soto, Semarhy; Reams, Andrew B.
Afiliación
  • Herrmann JA; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Koprowska A; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Winters TJ; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Villanueva N; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Nikityuk VD; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Pek F; Department of Mathematics and Statistics, California State University, Sacramento, CA 95819-6051, USA.
  • Reis EM; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Dominguez CZ; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Davis D; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • McPherson E; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Rocco SR; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Recendez C; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Difuntorum SM; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Faeth K; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Lopez MD; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Awwad HM; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Ghobashy RA; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Cappiello L; Department of Mathematics and Statistics, California State University, Sacramento, CA 95819-6051, USA.
  • Neidle EL; Department of Microbiology, University of Georgia, Athens, GA 30602-2605, USA.
  • Quiñones-Soto S; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
  • Reams AB; Department of Biological Sciences, California State University, Sacramento, CA 95819-6077, USA.
G3 (Bethesda) ; 13(3)2023 03 09.
Article en En | MEDLINE | ID: mdl-36504387
The controversial theory of adaptive amplification states gene amplification mutations are induced by selective environments where they are enriched due to the stress caused by growth restriction on unadapted cells. We tested this theory with three independent assays using an Acinetobacter baylyi model system that exclusively selects for cat gene amplification mutants. Our results demonstrate all cat gene amplification mutant colonies arise through a multistep process. While the late steps occur during selection exposure, these mutants derive from low-level amplification mutant cells that form before growth-inhibiting selection is imposed. During selection, these partial mutants undergo multiple secondary steps generating higher amplification over several days to multiple weeks to eventually form visible high-copy amplification colonies. Based on these findings, amplification in this Acinetobacter system can be explained by a natural selection process that does not require a stress response. These findings have fundamental implications to understanding the role of growth-limiting selective environments on cancer development. We suggest duplication mutations encompassing growth factor genes may serve as new genomic biomarkers to facilitate early cancer detection and treatment, before high-copy amplification is attained.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acinetobacter / Neoplasias Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acinetobacter / Neoplasias Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido