Your browser doesn't support javascript.
loading
The Microalga Skeletonema marinoi Induces Apoptosis and DNA Damage in K562 Cell Line by Modulating NADPH Oxidase.
Ciarcia, Roberto; Longobardi, Consiglia; Ferrara, Gianmarco; Montagnaro, Serena; Andretta, Emanuela; Pagnini, Francesco; Florio, Salvatore; Maruccio, Lucianna; Lauritano, Chiara; Damiano, Sara.
Afiliación
  • Ciarcia R; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Longobardi C; Department of Mental, Physical Health and Preventive Medicine, University of Campania "Luigi Vanvitelli", Largo Madonna delle Grazie n.1, 80138 Naples, Italy.
  • Ferrara G; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Montagnaro S; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Andretta E; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Pagnini F; Department of Medicine and Surgery, Unit of Radiology, University of Parma, Via Università n. 12, 43126 Parma, Italy.
  • Florio S; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Maruccio L; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
  • Lauritano C; Ecosustainable Marine Biotechnology Department, Stazione Zoologica Anton Dohrn, Via Acton n. 55, 80133 Naples, Italy.
  • Damiano S; Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", Via Delpino n.1, 80137 Naples, Italy.
Molecules ; 27(23)2022 Nov 27.
Article en En | MEDLINE | ID: mdl-36500363
Chronic myeloid leukemia (CML) is a myeloproliferative disease that activates multiple signaling pathways, causing cells to produce higher levels of reactive oxygen species (ROS). Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are a major generator of ROS in leukemia, and marine natural products have shown promising activities for the treatment of hematopoietic malignancies. In the present study, we investigated the effect of the marine microalga Skeletonema marinoi (S.M.), a ubiquitous diatom that forms massive blooms in the oceans, on the human leukemia cell line K562. The effects of S.M. extract on cell viability, production of ROS, nitric oxide (NO), and apoptosis were examined. In this preliminary work, S.M. was able to decrease cell viability (p < 0.05) and increase apoptosis levels (p < 0.05) in K562 cells after 48 h of treatment. In addition, the levels of NOX, NO, and malondialdehyde (MDA) were reduced in K562-treated cells (p < 0.05), whereas the levels of SOD, CAT, and GPx increased during treatment (p < 0.05). Finally, analyzing Bax and Bcl-2 expression, we found a significant increase in the proapoptotic protein Bax and a sustained decrease in the antiapoptotic protein Bcl-2 (p < 0.05) in the K562-treated cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mielógena Crónica BCR-ABL Positiva / Diatomeas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mielógena Crónica BCR-ABL Positiva / Diatomeas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza