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Development of a Cryopreservation Technique for Xenogeneic Kidney Grafts: Evaluation Using a Mouse Model.
Takamura, Tsuyoshi; Nagashima, Hiroshi; Matsunari, Hitomi; Yamanaka, Shuichiro; Saito, Yatsumu; Kinoshita, Yoshitaka; Fujimoto, Toshinari; Matsumoto, Kei; Nakano, Kazuaki; Okano, Hirotaka James; Kobayashi, Eiji; Yokoo, Takashi.
Afiliación
  • Takamura T; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Nagashima H; Meiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, Japan.
  • Matsunari H; PorMedTec Co., Ltd., Kawasaki 214-0034, Japan.
  • Yamanaka S; Meiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, Japan.
  • Saito Y; PorMedTec Co., Ltd., Kawasaki 214-0034, Japan.
  • Kinoshita Y; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Fujimoto T; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Matsumoto K; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Nakano K; Department of Urology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8654, Japan.
  • Okano HJ; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Kobayashi E; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
  • Yokoo T; Meiji University International Institute for Bio-Resource Research, Kawasaki 214-8571, Japan.
J Clin Med ; 11(23)2022 Dec 06.
Article en En | MEDLINE | ID: mdl-36498811
To align the xeno-metanephros and renal progenitor cell timing for transplantation treatments, cryopreservation techniques and an efficient transportation of regenerated renal products such as xeno-metanephroi and renal progenitor cells should be established. Therefore, we propose a novel method of xenogeneic regenerative medicine for patients with chronic kidney disease by grafting porcine fetal kidneys injected with human renal progenitor cells. To develop a useful cryopreserve system of porcine fetal kidney and human renal progenitor cells, we examined the cryopreservation of a fetal kidney implanted with renal progenitor cells in a mouse model. First, we developed a new method for direct cell injection under the capsule of the metanephros using gelatin as a support for unzipped fetal kidneys. Then, we confirmed in vitro that the nephrons derived from the transplanted cells were regenerated even after cryopreservation before and after cell transplantation. Furthermore, the cryopreserved chimeric metanephroi grew, and regenerated nephrons were observed in NOD. We confirmed that even in cryopreserved chimeric metanephroi, transplanted cell-derived nephrons as well as fresh transplants grew.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Clin Med Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza