Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene.
Cell
; 50(3): 435-43, 1987 Jul 31.
Article
en En
| MEDLINE
| ID: mdl-3649277
A method of deleting specific cell lineages has been developed that entails microinjection into fertilized eggs of a chimeric gene in which a cell-specific enhancer/promoter is used to drive the expression of a toxic gene product. We show that microinjection of a construct in which the elastase I promoter/enhancer is fused to a gene for diphtheria toxin A polypeptide results in birth of mice lacking a normal pancreas because of expression of the toxin in pancreatic acinar cells. A small pancreatic rudiment, containing islet and duct-like cells, was observed in some of the transgenic mice. This method provides a new approach for studying cell-lineage relationships and for analyzing cellular interactions during development.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Recombinante
/
Regulación de la Expresión Génica
/
Genes Reguladores
/
Elementos de Facilitación Genéticos
/
Toxina Diftérica
Límite:
Animals
Idioma:
En
Revista:
Cell
Año:
1987
Tipo del documento:
Article
Pais de publicación:
Estados Unidos