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Discrete localization of phospholipase Cß3 and diacylglycerol kinase ι along the renal proximal tubules of normal rat kidney and gentamicin-induced changes in their expression.
Hemha, Premrudee; Chomphoo, Surang; Polsan, Yada; Goto, Kaoru; Watanabe, Masahiko; Kondo, Hisatake; Hipkaeo, Wiphawi.
Afiliación
  • Hemha P; Electron Microscopy Laboratory, Division of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Chomphoo S; Electron Microscopy Laboratory, Division of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Polsan Y; Electron Microscopy Laboratory, Division of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Goto K; Department of Anatomy, School of Medicine, Yamagata University, Yamagata, Japan.
  • Watanabe M; Department of Anatomy, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
  • Kondo H; Electron Microscopy Laboratory, Division of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
  • Hipkaeo W; Electron Microscopy Laboratory, Division of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. wiphawi@kku.ac.th.
Histochem Cell Biol ; 159(3): 293-307, 2023 Mar.
Article en En | MEDLINE | ID: mdl-36478081
Many signaling enzymes have multiple isozymes that are localized discretely at varying molecular levels in different compartments of cells where they play specific roles. In this study, among the various isozymes of phospholipase C (PLC) and diacylglycerol kinase (DGK), which work sequentially in the phosphoinositide cycle, both PLCß3 and DGKι were found in renal brush-border microvilli, but found to replace each other along the proximal tubules: PLCß3 in the proximal straight tubules (PST) of the outer stripe of the outer medulla (OSOM) and the medullary ray (MR), and DGKι in the proximal convoluted tubules (PCT) in the cortex and partially in the PST of the MR. Following daily injection of gentamicin for 1 week, the expression of PLCß3 and DGKι was transiently enhanced, as demonstrated by western blot, and the increases were found to most likely occur in their original sites, that is, in the brush borders of the PST for PLCß3 and in the PCT for DGKι. These findings showing differences in expression along the tubules suggest that the exertion of reabsorption and secretion through various ion channels and transporters in the microvillus membranes and the maintenance of microvillus turnover are regulated by a PLC-mediated signal with the balance shifted toward relative augmentation of the DAG function in the PST, and by a DGK-mediated signal with the balance shifted to relative augmentation of the phosphatidic acid function in the PCT. Our results also suggest the possibility that these isozymes are potential diagnostic signs for the early detection of acute kidney injury caused by gentamicin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolipasas / Diacilglicerol Quinasa Tipo de estudio: Screening_studies Límite: Animals Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfolipasas / Diacilglicerol Quinasa Tipo de estudio: Screening_studies Límite: Animals Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Alemania