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In vitro production of infectious Plasmodium falciparum sporozoites.
Eappen, Abraham G; Li, Tao; Marquette, Meghan; Chakravarty, Sumana; Kc, Natasha; Zanghi, Gigliola; Hoffman, Benjamin U; Hettiarachchi, Hashani; Patil, Asha; Abebe, Yonas; Tran, Christiane; Yossef, Alemtaye A; McWilliams, Ian; Morrison, Robert D; Rathakrishnan, Ayyappan; Inbar, Ehud; Aly, Ahmed S I; De La Vega, Patricia; Belmonte, Maria; Sedegah, Martha; Wai, Tint; Campo, Joseph J; King, Harley; Kappe, Stefan H I; Li, MingLin; Billingsley, Peter F; Sim, B Kim Lee; Hoffman, Stephen L.
Afiliación
  • Eappen AG; Sanaria, Rockville, MD, USA.
  • Li T; Sanaria, Rockville, MD, USA.
  • Marquette M; Sanaria, Rockville, MD, USA.
  • Chakravarty S; Sanaria, Rockville, MD, USA.
  • Kc N; Sanaria, Rockville, MD, USA.
  • Zanghi G; Protein Potential, Rockville, MD, USA.
  • Hoffman BU; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.
  • Hettiarachchi H; Columbia University Irving Medical Center, New York, NY, USA.
  • Patil A; Department of Medicine, UCSF, San Francisco, CA, USA.
  • Abebe Y; Sanaria, Rockville, MD, USA.
  • Tran C; Heritage College of Osteopathic Medicine, Ohio University, Dublin, OH, USA.
  • Yossef AA; Sanaria, Rockville, MD, USA.
  • McWilliams I; Sanaria, Rockville, MD, USA.
  • Morrison RD; Sanaria, Rockville, MD, USA.
  • Rathakrishnan A; Sanaria, Rockville, MD, USA.
  • Inbar E; Sanaria, Rockville, MD, USA.
  • Aly ASI; Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, USA.
  • De La Vega P; Sanaria, Rockville, MD, USA.
  • Belmonte M; Sanaria, Rockville, MD, USA.
  • Sedegah M; Sanaria, Rockville, MD, USA.
  • Wai T; Sanaria, Rockville, MD, USA.
  • Campo JJ; Malaria Department, Naval Medical Research Center, Silver Spring, MD, USA.
  • King H; Henry M. Jackson Foundation, Bethesda, MD, USA.
  • Kappe SHI; Malaria Department, Naval Medical Research Center, Silver Spring, MD, USA.
  • Li M; Sanaria, Rockville, MD, USA.
  • Billingsley PF; Protein Potential, Rockville, MD, USA.
  • Sim BKL; Antigen Discovery Incorporated (ADI), Irvine, CA, USA.
  • Hoffman SL; Institute for Bioscience and Biotechnology Research, Rockville, MD, USA.
Nature ; 612(7940): 534-539, 2022 12.
Article en En | MEDLINE | ID: mdl-36477528
An effective vaccine is needed for the prevention and elimination of malaria. The only immunogens that have been shown to have a protective efficacy of more than 90% against human malaria are Plasmodium falciparum (Pf) sporozoites (PfSPZ) manufactured in mosquitoes (mPfSPZ)1-7. The ability to produce PfSPZ in vitro (iPfSPZ) without mosquitoes would substantially enhance the production of PfSPZ vaccines and mosquito-stage malaria research, but this ability is lacking. Here we report the production of hundreds of millions of iPfSPZ. iPfSPZ invaded human hepatocytes in culture and developed to mature liver-stage schizonts expressing P. falciparum merozoite surface protein 1 (PfMSP1) in numbers comparable to mPfSPZ. When injected into FRGhuHep mice containing humanized livers, iPfSPZ invaded the human hepatocytes and developed to PfMSP1-expressing late liver stage parasites at 45% the quantity of cryopreserved mPfSPZ. Human blood from FRGhuHep mice infected with iPfSPZ produced asexual and sexual erythrocytic-stage parasites in culture, and gametocytes developed to PfSPZ when fed to mosquitoes, completing the P. falciparum life cycle from infectious gametocyte to infectious gametocyte without mosquitoes or primates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Esporozoítos Límite: Animals / Humans Idioma: En Revista: Nature Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Esporozoítos Límite: Animals / Humans Idioma: En Revista: Nature Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido