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Ultrasensitive photoelectrochemical biosensor for DNA 5-methylcytosine analysis based on co-sensitization strategy combined with bridged DNA nanoprobe.
Zhu, Chuiyu; Tian, Xiao; Li, Qiuyan; Dai, Zichang; Wang, Lina; Liu, Huamin; Li, Chenghong; Zahid, Kashif Rafiq; Wu, Chunmei; Huang, Hui; Fang, Lichao; Huang, Qiyuan; Liu, Feixue; Deng, Jun; Li, Yan; Zeng, Tao; Zheng, Junsong.
Afiliación
  • Zhu C; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China; Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, China.
  • Tian X; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Li Q; Laboratory Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524002, China.
  • Dai Z; Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, China.
  • Wang L; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Liu H; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Li C; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Zahid KR; Laboratory Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524002, China.
  • Wu C; Laboratory Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524002, China.
  • Huang H; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Fang L; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Huang Q; Laboratory Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524002, China.
  • Liu F; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Deng J; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China.
  • Li Y; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China. Electronic address: liyanlwb1003@126.com.
  • Zeng T; Laboratory Medicine Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524002, China; Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, China. Electronic address: zengt@smu.edu.cn.
  • Zheng J; Department of Clinical and Military Laboratory Medicine, Army Medical University, Chongqing, 400038, China. Electronic address: zhengalpha@sina.com.
Talanta ; 254: 124140, 2023 Mar 01.
Article en En | MEDLINE | ID: mdl-36463802
Altered DNA methylation in the form of 5-methylcytosine (5-mC) patterns is correlated with disease diagnosis, prognosis, and treatment response. Therefore, accurate analysis of 5-mC is of great significance for the diagnosis of diseases. Here, an efficient enhanced photoelectrochemical (PEC) biosensor was designed for the quantitative analysis of DNA 5-mC based on a cascaded energy level aligned co-sensitization strategy coupling with the bridged DNA nanoprobe (BDN). Firstly, Au nanoparticle/graphite phase carbon nitride/titanium dioxide (AuNPs/g-C3N4@TiO2) nanocomposite was synthesized through in situ growth of AuNPs on g-C3N4@TiO2 surface as a matrix to provide a stable background signal. Next, BDN with a high mass transfer rate synthesized from a pair of DNA tetrahedral as nanomechanical handles was used as a capture probe to bind to the target sequence. The polydopamine nanosphere was applied to load with CdTe QDs (PDANS-CdTe QDs) as a photocurrent label of 5-mC antibodies. When the 5-mC existed, a large number of PDANS-Ab-CdTe QDs were introduced to the electrode surface, the formed CdTe QDs/AuNPs/g-C3N4@TiO2 co-sensitive structure could effectively enhance the electron transfer capability and photocurrent response rate due to the effective cascade energy level arrangement, leading to a significantly enhanced photocurrent signal. The proposed PEC biosensor manifested a wide range from 10-17 M to 10-7 M and a detection limit of 2.2 aM. Meanwhile, the excellent performance indicated the practicability of the designed strategy, thus being capable of the clinical diagnosis of 5-mC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Compuestos de Cadmio / Puntos Cuánticos / Nanopartículas del Metal Idioma: En Revista: Talanta Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Compuestos de Cadmio / Puntos Cuánticos / Nanopartículas del Metal Idioma: En Revista: Talanta Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos