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Matrix Metalloproteinase 1 as a Marker of Tonsil-Derived Mesenchymal Stem Cells to Assess Bone Marrow Cell Migration.
Kim, Hee-Yeon; Yoon, Hee-Soo; Lee, Younghay; Kim, Yu-Hee; Cho, Kyung-Ah; Woo, So-Youn; Kim, Han Su; Ryu, Kyung-Ha; Park, Joo-Won.
Afiliación
  • Kim HY; Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Yoon HS; Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Lee Y; Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Kim YH; Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Cho KA; Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Woo SY; Department of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
  • Kim HS; Department of Otolaryngology, College of Medicine, Ewha Womans University, Seoul, 07985, South Korea.
  • Ryu KH; Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea. ykh@ewha.ac.kr.
  • Park JW; Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea. joowon.park@ewha.ac.kr.
Tissue Eng Regen Med ; 20(2): 271-284, 2023 04.
Article en En | MEDLINE | ID: mdl-36462090
BACKGROUND: To achieve optimal bone marrow engraftment during bone marrow transplantation, migration of donor bone marrow cells (BMCs) toward the recipient's bone marrow is critical. Despite the enhanced engraftment of BMCs by co-administration of mesenchymal stem cells (MSCs), the efficiency can be variable depending on MSC donor. The purpose of this study is to examine the functional heterogeneity of tonsil-derived MSCs (TMSCs) and to identify a marker to evaluate efficacy for the enhancement of BMC migration. METHODS: To examine the donor-to-donor variation of TMSCs in potentiating BMC migration, we isolated TMSCs from 25 independent donors. Transcriptome of TMSCs and proteome of conditioned medium derived from TMSC were analyzed. RESULTS: Enhanced BMC migration by conditioned medium derived from TMSCs was variable depending on TMSC donor. The TMSCs derived from 25 donors showed distinct expression profiles compared with other cells, including fibroblasts, adipose-derived MSCs and bone marrow-derived MSCs. TMSCs were distributed in two categories: high- and low-efficacy groups for potentiating BMC migration. Transcriptome analysis of TMSCs and proteome profiles of conditioned medium derived from TMSCs revealed higher expression and secretion of matrix metalloproteinase (MMP) 1 in the high-efficacy group. MMP1 knockdown in TMSCs abrogated the supportive efficacy of conditioned medium derived from TMSC cultures in BMC migration. CONCLUSION: These data suggest that secreted MMP1 can be used as a marker to evaluate the efficacy of TMSCs in enhancing BMC migration. Furthermore, the strategy of analyzing transcriptomes and proteomes of the MSCs may be useful to set the standard for donor variation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tonsila Palatina / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Tissue Eng Regen Med Año: 2023 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Corea del Sur
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tonsila Palatina / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Tissue Eng Regen Med Año: 2023 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Corea del Sur