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Widespread, human-associated redondoviruses infect the commensal protozoan Entamoeba gingivalis.
Keeler, Emma L; Merenstein, Carter; Reddy, Shantan; Taylor, Louis J; Cobián-Güemes, Ana G; Zankharia, Urvi; Collman, Ronald G; Bushman, Frederic D.
Afiliación
  • Keeler EL; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Merenstein C; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Reddy S; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Taylor LJ; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
  • Cobián-Güemes AG; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Zankharia U; Department of Medicine, Pulmonary, Allergy and Critical Care Division, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Collman RG; Department of Medicine, Pulmonary, Allergy and Critical Care Division, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: collmanr@pennmedicine.upenn.edu.
  • Bushman FD; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address: bushman@pennmedicine.upenn.edu.
Cell Host Microbe ; 31(1): 58-68.e5, 2023 01 11.
Article en En | MEDLINE | ID: mdl-36459997
Redondoviruses are circular Rep-encoding single-stranded DNA (CRESS) viruses of high prevalence in healthy humans. Redondovirus abundance is increased in oro-respiratory samples from individuals with periodontitis, acute illness, and severe COVID-19. We investigated potential host cells supporting redondovirus replication in oro-respiratory samples and uncovered the oral amoeba Entamoeba gingivalis as a likely host. Redondoviruses are closely related to viruses of Entamoeba and contain reduced GC nucleotide content, consistent with Entamoeba hosts. Redondovirus and E. gingivalis co-occur in metagenomic data from oral disease and healthy human cohorts. When grown in xenic cultures with feeder bacteria, E. gingivalis was robustly positive for redondovirus RNA and DNA. A DNA proximity-ligation assay (Hi-C) on xenic culture cells showed enriched cross-linking of redondovirus and Entamoeba DNA, supporting E. gingivalis as the redondovirus host. While bacteria are established hosts for bacteriophages within the human virome, this work shows that eukaryotic commensals also contribute an abundant human-associated virus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Bacteriófagos / Virus / Entamoeba / COVID-19 Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Bacteriófagos / Virus / Entamoeba / COVID-19 Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Host Microbe Asunto de la revista: MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos