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Antimicrobial peptide-grafted PLGA-PEG nanoparticles to fight bacterial wound infections.
Ramôa, António Miguel; Campos, Filipa; Moreira, Luís; Teixeira, Cátia; Leiro, Victoria; Gomes, Paula; das Neves, José; Martins, M Cristina L; Monteiro, Cláudia.
Afiliación
  • Ramôa AM; i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. claudia.monteiro@ineb.up.pt.
  • Campos F; INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.
  • Moreira L; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
  • Teixeira C; Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal.
  • Leiro V; i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. claudia.monteiro@ineb.up.pt.
  • Gomes P; INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.
  • das Neves J; i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. claudia.monteiro@ineb.up.pt.
  • Martins MCL; INEB, Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.
  • Monteiro C; Escola Superior de Biotecnologia, Universidade Católica do Porto, Rua de Diogo Botelho, 1327, 4169-005 Porto, Portugal.
Biomater Sci ; 11(2): 499-508, 2023 Jan 17.
Article en En | MEDLINE | ID: mdl-36458466
Wound infection treatment with antimicrobial peptides (AMPs) is still not a reality, due to the loss of activity in vivo. Unlike the conventional strategy of encapsulating AMPs on nanoparticles (NPs) leaving activity dependent on the release profile, this work explores AMP grafting to poly(D,L-lactide-co-glycolide)-polyethylene glycol NPs (PLGA-PEG NPs), whereby AMP exposition, infection targeting and immediate action are promoted. NPs are functionalized with MSI-78(4-20), an equipotent and more selective derivative of MSI-78, grafted through a thiol-maleimide (Mal) Michael addition. NPs with different ratios of PLGA-PEG/PLGA-PEG-Mal are produced and characterized, with 40%PLGA-PEG-Mal presenting the best colloidal properties and higher amounts of AMP grafted as shown by surface charge (+8.6 ± 1.8 mV) and AMP quantification (326 µg mL-1, corresponding to 16.3 µg of AMP per mg of polymer). NPs maintain the activity of the free AMP with a minimal inhibitory concentration (MIC) of 8-16 µg mL-1 against Pseudomonas aeruginosa, and 16-32 µg mL-1 against Staphylococcus aureus. Moreover, AMP grafting accelerates killing kinetics, from 1-2 h to 15 min for P. aeruginosa and from 6-8 h to 0.5-1 h for S. aureus. NP activity in a simulated wound fluid is maintained for S. aureus and decreases slightly for P. aeruginosa. Furthermore, NPs do not demonstrate signs of cytotoxicity at MIC concentrations. Overall, this promising formulation helps unleash the full potential of AMPs for the management of wound infections.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Péptidos Antimicrobianos Idioma: En Revista: Biomater Sci Año: 2023 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas / Péptidos Antimicrobianos Idioma: En Revista: Biomater Sci Año: 2023 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido